Recruitment of Polo-like kinase couples synapsis to meiotic progression via inactivation of CHK-2

Zhang, Liangyu; Stauffer, Weston T.; Wang, John S.; Wu, Fan; Yu, Zhouliang; Liu, Chenshu; Kim, Hyung Jun; Dernburg, Abby F.

doi:10.7554/elife.84492

Recruitment of Polo-like kinase couples synapsis to meiotic progression via inactivation of CHK-2

Journal Article · Thu Jan 26 00:00:00 EST 2023 · eLife

DOI:https://doi.org/10.7554/elife.84492· OSTI ID:2470671

^[1]; ^[2]; Wang, John S. ^[3]; Wu, Fan ^[3]; Yu, Zhouliang ^[1]; Liu, Chenshu ^[3]; Kim, Hyung Jun ^[4]; ^[1]

University of California, Berkeley, CA (United States); Howard Hughes Medical Inst., Chevy Chase, MD (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Howard Hughes Medical Inst., Chevy Chase, MD (United States); University of California, Berkeley, CA (United States)
University of California, Berkeley, CA (United States); Howard Hughes Medical Inst., Chevy Chase, MD (United States)
University of California, Berkeley, CA (United States)

Meiotic chromosome segregation relies on synapsis and crossover (CO) recombination between homologous chromosomes. These processes require multiple steps that are coordinated by the meiotic cell cycle and monitored by surveillance mechanisms. In diverse species, failures in chromosome synapsis can trigger a cell cycle delay and/or lead to apoptosis. How this key step in ‘homolog engagement’ is sensed and transduced by meiotic cells is unknown. Here we report that in C. elegans, recruitment of the Polo-like kinase PLK-2 to the synaptonemal complex triggers phosphorylation and inactivation of CHK-2, an early meiotic kinase required for pairing, synapsis, and double-strand break (DSB) induction. Inactivation of CHK-2 terminates DSB formation and enables CO designation and cell cycle progression. These findings illuminate how meiotic cells ensure CO formation and accurate chromosome segregation.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2470671

Journal Information:: eLife, Journal Name: eLife Vol. 12; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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