Transcript and blood-microbiome analysis towards a blood diagnostic tool for goats affected by Haemonchus contortus

Tilahun, Yonathan; Pinango, Jessica Quijada; Johnson, Felicia; Lett, Charles; Smith, Kayla; Gipson, Terry; McCallum, Malcolm; Hoyt, Peter; Tritt, Andrew; Yadav, Archana; Elshahed, Mostafa; Wang, Zaisen

doi:10.1038/s41598-022-08939-x

Transcript and blood-microbiome analysis towards a blood diagnostic tool for goats affected by Haemonchus contortus

Journal Article · Wed Mar 30 00:00:00 EDT 2022 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-022-08939-x· OSTI ID:2470667

Tilahun, Yonathan ^[1]; Pinango, Jessica Quijada ^[1]; Johnson, Felicia ^[1]; Lett, Charles ^[1]; Smith, Kayla ^[1]; Gipson, Terry ^[1]; McCallum, Malcolm ^[1]; Hoyt, Peter ^[2]; Tritt, Andrew ^[3]; Yadav, Archana ^[2]; Elshahed, Mostafa ^[2]; Wang, Zaisen ^[1]

Langston Univ., OK (United States)
Oklahoma State Univ., Stillwater, OK (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

The Alpine goat (Capra aegagrus hircus) is parasitized by the barber pole worm (Haemonchus contortus). Hematological parameters from transcript and metagenome analysis in the host are reflective of infestation. We explored comparisons between blood samples of control, infected, infected zoledronic acid-treated, and infected antibody (anti-γδ T cells) treated wethers under controlled conditions. Seven days post-inoculation (dpi), we identified 7,627 transcripts associated with the different treatment types. Microbiome measurements at 7 dpi revealed fewer raw read counts across all treatments and a less diverse microbial flora than at 21 dpi. This study identifies treatment specific transcripts and an increase in microflora abundance and diversity as wethers age. Further, F/B ratio reflect health, based on depression or elevation above thresholds defined by the baseline of non-infected controls. Forty Alpine wethers were studied where blood samples were collected from five goats in four treatment groups on 7 dpi and 21 dpi. Transcript and microbiome profiles were obtained using the Partek Flow (St. Louis, Missouri, USA) software suites pipelines. Inflammation comparisons were based on the Firmicutes/Bacteriodetes ratios that are calculated as well as the reduction of microbial diversity.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: US Dept. of Agriculture (USDA); USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2470667

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 12; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Transcript and blood-microbiome analysis towards a blood diagnostic tool for goats affected by Haemonchus contortus

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