The tubulin structure, a quarter of a century later

Nogales, Eva

doi:10.1091/mbc.e23-01-0005

The tubulin structure, a quarter of a century later

Journal Article · Mon Mar 13 00:00:00 EDT 2023 · Molecular Biology of the Cell

DOI:https://doi.org/10.1091/mbc.e23-01-0005· OSTI ID:2470623

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University of California, Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

This retrospective on the 25th anniversary of the publication of the first structure of tubulin is shaped by my own personal experiences rather than being a strict and complete historical account of the event. It is a reflection on how working in science felt many years ago, on the struggles and the joys of reaching for the high-hanging fruit, and, ultimately, on how relevant or not our personal scientific contributions are to the broader scientific community. Writing it brought back memories of my unique and sadly lost postdoctoral advisor Ken Downing, who dreamt of this structure and brought it to fruition against all odds.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2470623

Journal Information:: Molecular Biology of the Cell, Journal Name: Molecular Biology of the Cell Journal Issue: 4 Vol. 34; ISSN 1059-1524

Publisher:: American Society for Cell BiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (19)

Refined structure of αβ-tubulin at 3.5 Å resolution 1 1Edited by I. A. Wilson Löwe, J.; Li, H.; Downing, K. H. Journal of Molecular Biology, Vol. 313, Issue 5 https://doi.org/10.1006/jmbi.2001.5077	journal	November 2001
Induction of a sheet polymer of tubulin by Zn2+ Larsson, H.; Wallin, Margareta; Edström, A. Experimental Cell Research, Vol. 100, Issue 1 https://doi.org/10.1016/0014-4827(76)90332-3	journal	June 1976
Structure of the tubulin dimer in zinc-induced sheets Baker, T. S.; Amos, L. A. Journal of Molecular Biology, Vol. 123, Issue 1 https://doi.org/10.1016/0022-2836(78)90378-9	journal	July 1978
Characterization of microtubule protofilament numbers Wade, R. H.; Chrétien, D.; Job, D. Journal of Molecular Biology, Vol. 212, Issue 4 https://doi.org/10.1016/0022-2836(90)90236-F	journal	April 1990
Projection map of tubulin in zinc-induced sheets at 4 Å resolution Downing, Kenneth H.; Jontes, James Journal of Structural Biology, Vol. 109, Issue 2 https://doi.org/10.1016/1047-8477(92)90046-D	journal	September 1992
Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy Henderson, R.; Baldwin, J. M.; Ceska, T. A. Journal of Molecular Biology, Vol. 213, Issue 4 https://doi.org/10.1016/S0022-2836(05)80271-2	journal	June 1990
The 4 Å X-Ray Structure of a Tubulin:Stathmin-like Domain Complex Gigant, Benoît; Curmi, Patrick A.; Martin-Barbey, Carole Cell, Vol. 102, Issue 6 https://doi.org/10.1016/S0092-8674(00)00069-6	journal	September 2000
High-Resolution Model of the Microtubule Nogales, Eva; Whittaker, Michael; Milligan, Ronald A. Cell, Vol. 96, Issue 1 https://doi.org/10.1016/S0092-8674(00)80961-7	journal	January 1999
High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis Alushin, Gregory M.; Lander, Gabriel C.; Kellogg, Elizabeth H. Cell, Vol. 157, Issue 5 https://doi.org/10.1016/j.cell.2014.03.053	journal	May 2014
Structure of the Decorated Ciliary Doublet Microtubule Ma, Meisheng; Stoyanova, Mihaela; Rademacher, Griffin Cell, Vol. 179, Issue 4 https://doi.org/10.1016/j.cell.2019.09.030	journal	October 2019
Structure of the αβ tubulin dimer by electron crystallography Nogales, Eva; Wolf, Sharon G.; Downing, Kenneth H. Nature, Vol. 391, Issue 6663 https://doi.org/10.1038/34465	journal	January 1998
Crystal structure of the bacterial cell-division protein FtsZ Löwe, Jan; Amos, Linda A. Nature, Vol. 391, Issue 6663 https://doi.org/10.1038/34472	journal	January 1998
Atomic structure of the actin: DNase I complex Kabsch, Wolfgang; Mannherz, Hans Georg; Suck, Dietrich Nature, Vol. 347, Issue 6288 https://doi.org/10.1038/347037a0	journal	September 1990
Atomic model of the actin filament Holmes, Kenneth C.; Popp, David; Gebhard, Werner Nature, Vol. 347, Issue 6288 https://doi.org/10.1038/347044a0	journal	September 1990
Structure of tubulin at 6.5 Å and location of the taxol-binding site Nogales, Eva; Grayer Wolf, Sharon; Khan, Israr A. Nature, Vol. 375, Issue 6530 https://doi.org/10.1038/375424a0	journal	June 1995
Crystal structure of the kinesin motor domain reveals a structural similarity to myosin Jon Kull, F.; Sablin, Elena P.; Lau, Rebecca Nature, Vol. 380, Issue 6574 https://doi.org/10.1038/380550a0	journal	April 1996
Electron Diffraction of Frozen, Hydrated Protein Crystals Taylor, K. A.; Glaeser, R. M. Science, Vol. 186, Issue 4168 https://doi.org/10.1126/science.186.4168.1036	journal	December 1974
Three-dimensional structure of myosin subfragment-1: a molecular motor Rayment, I.; Rypniewski, W.; Schmidt-Base, K. Science, Vol. 261, Issue 5117 https://doi.org/10.1126/science.8316857	journal	July 1993
Structure of the actin-myosin complex and its implications for muscle contraction Rayment, I.; Holden, H.; Whittaker, M. Science, Vol. 261, Issue 5117 https://doi.org/10.1126/science.8316858	journal	July 1993

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