Binding of Cholera Toxin B-Subunit to a Ganglioside GM1-Functionalized PEG-Tethered Lipid Membrane

Watkins, Erik B.; Dennison, Andrew J.  C.; Majewski, Jaroslaw

doi:10.1021/acs.langmuir.2c00499

Binding of Cholera Toxin B-Subunit to a Ganglioside GM1-Functionalized PEG-Tethered Lipid Membrane

Journal Article · Mon May 23 00:00:00 EDT 2022 · Langmuir

DOI:https://doi.org/10.1021/acs.langmuir.2c00499· OSTI ID:2470474

^[1]; Dennison, Andrew J. C. ^[2]; Majewski, Jaroslaw ^[3]

Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); Institut Laue-Langevin, Grenoble (France)
University of Sheffield (United Kingdom)
National Science Foundation, Alexandria, VA (United States); Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); University of New Mexico, Albuquerque, NM (United States)

We report neutron reflectometry (NR) studies of polyethylene glycol (PEG)-tethered model lipid membranes at the solid–liquid interface and of cholera toxin’s B-subunit (CTxB) binding to tethered membranes containing ganglioside GM1 receptors. First, tethered polymer brushes were formed by grafting silane-functionalized PEG lipopolymers to quartz from solution. Subsequent deposition of lipids by Langmuir–Blodgett/Langmuir–Schaefer (LB/LS) resulted in a tethered bilayer structure separated from the solid support by a hydrated PEG layer. NR revealed that the tethers formed a highly hydrated polymer brush, uniformly separating the bilayer from the underlying solid substrate. Further, the lipid bilayer did not significantly perturb the brush’s conformation relative to a free brush. Biological functionality of the tethered bilayers was verified by interacting CTxB, with ganglioside GM1 receptors incorporated into the bilayer. The surface coverage of CTxB bound to the lipid membrane, θ_CTB= 0.58 ± 0.08, was consistent with the coverage predicted for random sequential absorption, and toxin binding did not impact the membrane conformation.

View Accepted Manuscript (DOE)

Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: 89233218CNA000001

OSTI ID:: 2470474

Journal Information:: Langmuir, Journal Name: Langmuir Journal Issue: 22 Vol. 38; ISSN 0743-7463

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Binding of Cholera Toxin B-Subunit to a Ganglioside GM1-Functionalized PEG-Tethered Lipid Membrane

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