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Botulinum Neurotoxin Detection and Differentiation by Mass Spectrometry

Journal Article · · Emerging Infectious Diseases
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  1. Centers for Disease Control and Prevention (CDC), Atlanta, GA (United States)
  2. Battelle Memorial Inst., Atlanta, GA (United States)
  3. Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
+ Show Author Affiliations
Botulinum neurotoxins (BoNTs) are proteases that cleave specific cellular proteins essential for neurotransmitter release. Seven BoNT serotypes (A–G) exist; 4 usually cause human botulism (A, B, E, and F). We developed a rapid, mass spectrometry–based method (Endopep-MS) to detect and differentiate active BoNTs A, B, E, and F. This method uses the highly specific protease activity of the toxins with target peptides specific for each toxin serotype. The product peptides derived from the endopeptidase activities of BoNTs are detected by matrix-assisted laser-desorption ionization time-of-flight mass spectrometry. In buffer, this method can detect toxin equivalents of as little as 0.01 mouse lethal dose (MLD)50 and concentrations as low as 0.62 MLD50/mL. A high-performance liquid chromatography–tandem mass spectrometry method for quantifying active toxin, where the amount of toxin can be correlated to the amount of product peptides, is also described.
Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
89233218CNA000001
OSTI ID:
2470429
Journal Information:
Emerging Infectious Diseases, Journal Name: Emerging Infectious Diseases Journal Issue: 10 Vol. 11; ISSN 1080-6040
Publisher:
Centers for Disease Control and Prevention (CDC)Copyright Statement
Country of Publication:
United States
Language:
English

References (14)

Botulinum Toxin as a Biological Weapon: Medical and Public Health Management journal February 2001
High-Throughput Fluorogenic Assay for Determination of Botulinum Type B Neurotoxin Protease Activity journal April 2001
High-Throughput Assays for Botulinum Neurotoxin Proteolytic Activity: Serotypes A, B, D, and F journal September 2001
Proteolysis of synthetic peptides by type A botulinum neurotoxin journal November 1995
Isotope dilution--mass spectrometric quantification of specific proteins: model application with apolipoprotein A-I journal October 1996
Detection of Botulinal Neurotoxins A, B, E, and F by Amplified Enzyme-Linked Immunosorbent Assay: Collaborative Study journal March 2003
Recombinant SINAP-25 is an effective substrate for Clostridium botulinum type A toxin endopeptidase activity in vitro journal October 1997
Peptide Substrate Specificity and Properties of the zinc‐endopeptidase Activity of Botulinum Type B Neurotoxin journal October 1994
Development of in vitro assays for the detection of botulinum toxins in foods journal July 1999
Development of an In Vitro Bioassay for Clostridium botulinum Type B Neurotoxin in Foods That Is More Sensitive than the Mouse Bioassay journal September 1999
Fluorigenic Substrates for the Protease Activities of Botulinum Neurotoxins, Serotypes A, B, and F journal January 2003
Development of novel assays for botulinum type A and B neurotoxins based on their endopeptidase activities journal August 1996
Neurotoxins Affecting Neuroexocytosis journal January 2000
Comparison of the Mouse Bioassay and Enzyme-Linked Immunosorbent Assay Procedures for the Detection of Type A Botulinal Toxin in Food journal January 2004

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
immunology
infectious diseases