The Complete Genome Sequence and Structure of the Oleaginous Rhodococcus opacus Strain PD630 Through Nanopore Technology

Firrincieli, Andrea; Grigoriev, Beatrice; Dostálová, Hana; Cappelletti, Martina

doi:10.3389/fbioe.2021.810571

The Complete Genome Sequence and Structure of the Oleaginous Rhodococcus opacus Strain PD630 Through Nanopore Technology

Journal Article · Wed Feb 16 23:00:00 EST 2022 · Frontiers in Bioengineering and Biotechnology

DOI:https://doi.org/10.3389/fbioe.2021.810571· OSTI ID:2469660

Firrincieli, Andrea ^[1]; Grigoriev, Beatrice ^[1]; Dostálová, Hana ^[2]; Cappelletti, Martina ^[1]

Univ. of Bologna (Italy)
Institute of Microbiology of the Czech Academy of Sciences, Prague (Czechia)

Rhodococcus bacterial strains are characterized by wide metabolic versatility and extraordinary resistance to environmental stresses. The high versatility and adaptability of Rhodococcus strains is partly related with large and complex genomes (up to 10.1 Mbp) including high genetic redundancy and the presence of several circular and linear (mega)plasmids, which harbour peculiar catabolic and biosynthetic genes. Within Rhodococcus genus, R. opacus strain PD630 is considered a model oleaginous strain for its ability to produce and accumulate lipids (mostly triacyglycerols, TAGs) using different carbon sources, including low-cost and renewable resources such as lignocellulose. Notably, under specific growth conditions, this strain is capable of accumulating up to 80% of its cellular dry weight in TAGs; that is a rare feature in the prokaryotic and eukaryotic kingdoms. Multi-omic approaches have been applied to obtain system-level information about metabolic and regulatory pathways involved in these biosynthetic processes. Novel molecular tools for genome editing (CRISPR/Cas9 and recombineering) have been recently developed highlighting the possible utilization of R. opacus PD630 as synthetic biology platform for lipids production.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2469660

Journal Information:: Frontiers in Bioengineering and Biotechnology, Journal Name: Frontiers in Bioengineering and Biotechnology Vol. 9; ISSN 2296-4185

Publisher:: Frontiers Media S.A.Copyright Statement

Country of Publication:: United States

Language:: English

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