Multigene deletions in lung adenocarcinomas from irradiated and control mice
Conference
·
OSTI ID:244586
- Argonne National Lab., IL (United States). Center for Mechanistic Biology and Biotechnology
K-ras codon 12 point mutations mRb and p53 gene deletions were examined in tissues from 120 normal lungs and lung adenocarcinomas that were Formalin-treated and paraffin-embedded 25 years ago. The results showed that 12 of 60 (20%) lung adenocarcinomas had mRb deletions. All lung adenocarcinomas that were initially found bearing deleted mRb had p53 deletions (15 of 15; 100%). A significantly higher mutation frequency for K-ras codon 12 point mutations was also found in the lung adenocarcinomas from mice exposed to 24 once-weekly neutron irradiation (10 of 10; 100%) compared with those exposed to 24 or 60 once-weekly {gamma}-ray doses (5 of 10; 50%). The data suggested that p53 and K-ras gene alterations were two contributory factors responsible for the increased incidence of lung adenocarcinoma in B6CF{sub 1} male mice exposed to protracted neutron radiation.
- Research Organization:
- Argonne National Lab., IL (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States)
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 244586
- Report Number(s):
- ANL/CMB/CP--90034; CONF-9604136--18; ON: DE96011121; CNN: Grant ES07141-02
- Country of Publication:
- United States
- Language:
- English
Similar Records
Rb and p53 gene deletions in lung adenocarcinomas from irradiated and control mice
Detection of codon 12 point mutations of K-ras gene from mouse lung adenocarcinoma by "enriched" PCR.
PCR detection of retinoblastoma gene deletions in radiation-induced mouse lung adenocarcinomas
Technical Report
·
Fri Aug 01 00:00:00 EDT 1997
·
OSTI ID:515531
Detection of codon 12 point mutations of K-ras gene from mouse lung adenocarcinoma by "enriched" PCR.
Journal Article
·
Wed Jul 01 00:00:00 EDT 1998
· Int. J. Radiat. Biol.
·
OSTI ID:938192
PCR detection of retinoblastoma gene deletions in radiation-induced mouse lung adenocarcinomas
Technical Report
·
Sun May 01 00:00:00 EDT 1994
·
OSTI ID:10149484