Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium

Liu, Helen W.; Urzica, Eugen I.; Gallaher, Sean D.; Schmollinger, Stefan; Blaby-Haas, Crysten E.; Iwai, Masakazu; Merchant, Sabeeha S.

doi:10.1007/s11120-024-01103-8

Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium

Journal Article · Wed Jul 17 00:00:00 EDT 2024 · Photosynthesis Research

DOI:https://doi.org/10.1007/s11120-024-01103-8· OSTI ID:3004859

Liu, Helen W. ^[1]; Urzica, Eugen I. ^[2]; Gallaher, Sean D. ^[3]; Schmollinger, Stefan ^[1]; Blaby-Haas, Crysten E. ^[2]; Iwai, Masakazu ^[4]; Merchant, Sabeeha S. ^[5]

University of California, Berkeley, CA (United States)
Univ. of California, Los Angeles, CA (United States)
Univ. of California, Los Angeles, CA (United States); University of California, Berkeley, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
University of California, Berkeley, CA (United States); Univ. of California, Los Angeles, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Low iron (Fe) bioavailability can limit the biosynthesis of Fe-containing proteins, which are especially abundant in photosynthetic organisms, thus negatively affecting global primary productivity. Understanding cellular coping mechanisms under Fe limitation is therefore of great interest. For this paper, we surveyed the temporal responses of Chlamydomonas (Chlamydomonas reinhardtii) cells transitioning from an Fe-rich to an Fe-free medium to document their short and long-term adjustments. While slower growth, chlorosis and lower photosynthetic parameters are evident only after one or more days in Fe-free medium, the abundance of some transcripts, such as those for genes encoding transporters and enzymes involved in Fe assimilation, change within minutes, before changes in intracellular Fe content are noticeable, suggestive of a sensitive mechanism for sensing Fe. Promoter reporter constructs indicate a transcriptional component to this immediate primary response. With acetate provided as a source of reduced carbon, transcripts encoding respiratory components are maintained relative to transcripts encoding components of photosynthesis and tetrapyrrole biosynthesis, indicating metabolic prioritization of respiration over photosynthesis. In contrast to the loss of chlorophyll, carotenoid content is maintained under Fe limitation despite a decrease in the transcripts for carotenoid biosynthesis genes, indicating carotenoid stability. These changes occur more slowly, only after the intracellular Fe quota responds, indicating a phased response in Chlamydomonas, involving both primary and secondary responses during acclimation to poor Fe nutrition.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of California, Berkeley, CA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: AC02-05CH11231; SC0020627

OSTI ID:: 3004859

Alternate ID(s):: OSTI ID: 2440838

Journal Information:: Photosynthesis Research, Journal Name: Photosynthesis Research Journal Issue: 3 Vol. 161; ISSN 0166-8595; ISSN 1573-5079

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium

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Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium

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