The reference genome and abiotic stress responses of the model perennial grass Brachypodium sylvaticum

Lei, Li; Gordon, Sean P.; Liu, Lifeng; Sade, Nir; Lovell, John T.; Rubio Wilhelmi, Maria Del Mar; Singan, Vasanth; Sreedasyam, Avinash; Hestrin, Rachel; Phillips, Jeremy; Hernandez, Bryan T.; Barry, Kerrie; Shu, Shengqiang; Jenkins, Jerry; Schmutz, Jeremy; Goodstein, David M.; Thilmony, Roger; Blumwald, Eduardo; Vogel, John P.; Hufford, ed., M.

doi:10.1093/g3journal/jkad245

The reference genome and abiotic stress responses of the model perennial grass Brachypodium sylvaticum

Journal Article · Thu Oct 26 00:00:00 EDT 2023 · G3

DOI:https://doi.org/10.1093/g3journal/jkad245· OSTI ID:2267598

; Gordon, Sean P.; Liu, Lifeng; ; ; Rubio Wilhelmi, Maria Del Mar; Singan, Vasanth; ; Hestrin, Rachel; Phillips, Jeremy; Hernandez, Bryan T.; Barry, Kerrie; ; ; ; Goodstein, David M.; ; ; ; Hufford, ed., M.

Abstract

Perennial grasses are important forage crops and emerging biomass crops and have the potential to be more sustainable grain crops. However, most perennial grass crops are difficult experimental subjects due to their large size, difficult genetics, and/or their recalcitrance to transformation. Thus, a tractable model perennial grass could be used to rapidly make discoveries that can be translated to perennial grass crops. Brachypodium sylvaticum has the potential to serve as such a model because of its small size, rapid generation time, simple genetics, and transformability. Here, we provide a high-quality genome assembly and annotation for B. sylvaticum, an essential resource for a modern model system. In addition, we conducted transcriptomic studies under 4 abiotic stresses (water, heat, salt, and freezing). Our results indicate that crowns are more responsive to freezing than leaves which may help them overwinter. We observed extensive transcriptional responses with varying temporal dynamics to all abiotic stresses, including classic heat-responsive genes. These results can be used to form testable hypotheses about how perennial grasses respond to these stresses. Taken together, these results will allow B. sylvaticum to serve as a truly tractable perennial model system.

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Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0001526; SC0008797; AC02-05CH11231

OSTI ID:: 2267598

Alternate ID(s):: OSTI ID: 2439381
OSTI ID: 2229004

Journal Information:: G3, Journal Name: G3 Journal Issue: 1 Vol. 14; ISSN 2160-1836

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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The reference genome and abiotic stress responses of the model perennial grass Brachypodium sylvaticum

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