Role of Microbes as Biocolloids in the Transport of Actinides from a Deep Underground Radioactive Waste Repository

Dodge, C J; Dunn, M; Francis, A J; Gillow, J B; Mantione, K; Pansoy-Hjelvik, M E; Papenguth, H W; Strietelmeier, B A

Title: Role of Microbes as Biocolloids in the Transport of Actinides from a Deep Underground Radioactive Waste Repository

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Abstract

We investigated the interaction of dissolved actinides Th, U, Np Zgpu, and Am, with a pure and a mixed culture of halophilic bactezia isolated from the Waste Isolation H.Iot Plant repository under anaerobic conditions to evaluate their potentiaI transport as biocolloids from the waste site. The sizes of the bacterial cells studied ranged from ().54 x 0.48 pm to 7.7 x 0.67pm Using sequential mimofiltration, we determined the ~~ation of actinides with fi-ee-living (mobile) bacterial cells suspended in a fluid medium containing. NaCl or M=W12 brine, at various phaes of their growth cycIes. The number of suspended kcteria rangy-d born 106 to 109 cells ml-*. Tine amount of actinide associatd with the wspend~ cell fraction (cakzdated & mol cell-*) was very Iow: Th, 10-*2; U, 10-1s - 10-lS; - ~ Np, 1o-15- 10-19; Pu, 10-ls -10-21 ; and h, 10-1* - 10-*9 ; and it varied with the bacteihl - CUIture studied. l%e differe&es in the asswiation are amibuted to the extent of bioamxmdation and biosorption by the bacteria pH, the compo&on of the brine, and the speziation and bioavaiIability of the actinides.

Authors:: Dodge, C J; Dunn, M; Francis, A J; Gillow, J B; Mantione, K; Pansoy-Hjelvik, M E; Papenguth, H W; Strietelmeier, B A

Publication Date:: 1998-12-17

Research Org.:: Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 2439

Report Number(s):: SAND98-0370J
ON: DE00002439

DOE Contract Number:: AC04-94AL85000

Resource Type:: Journal Article

Journal Name:: Radiochemeca Acta

Additional Journal Information:: Journal Name: Radiochemeca Acta

Country of Publication:: United States

Language:: English

Subject:: 05 NUCLEAR FUELS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; Microorganisms; Actinides; Radioactive Wastes

Citation Formats


                    Dodge, C J, Dunn, M, Francis, A J, Gillow, J B, Mantione, K, Pansoy-Hjelvik, M E, Papenguth, H W, and Strietelmeier, B A. Role of Microbes as Biocolloids in the Transport of Actinides from a Deep Underground Radioactive Waste Repository.  United States: N. p., 1998. 
        Web.

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                    Dodge, C J, Dunn, M, Francis, A J, Gillow, J B, Mantione, K, Pansoy-Hjelvik, M E, Papenguth, H W, & Strietelmeier, B A. Role of Microbes as Biocolloids in the Transport of Actinides from a Deep Underground Radioactive Waste Repository.  United States.

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                    Dodge, C J, Dunn, M, Francis, A J, Gillow, J B, Mantione, K, Pansoy-Hjelvik, M E, Papenguth, H W, and Strietelmeier, B A. 1998.  
        "Role of Microbes as Biocolloids in the Transport of Actinides from a Deep Underground Radioactive Waste Repository".  United States.  https://www.osti.gov/servlets/purl/2439.

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@article{osti_2439,

  title        = {Role of Microbes as Biocolloids in the Transport of Actinides from a Deep Underground Radioactive Waste Repository},

  author       = {Dodge, C J and Dunn, M and Francis, A J and Gillow, J B and Mantione, K and Pansoy-Hjelvik, M E and Papenguth, H W and Strietelmeier, B A},

  abstractNote = {We investigated the interaction of dissolved actinides Th, U, Np Zgpu, and Am, with a pure and a mixed culture of halophilic bactezia isolated from the Waste Isolation H.Iot Plant repository under anaerobic conditions to evaluate their potentiaI transport as biocolloids from the waste site. The sizes of the bacterial cells studied ranged from ().54 x 0.48 pm to 7.7 x 0.67pm Using sequential mimofiltration, we determined the ~~ation of actinides with fi-ee-living (mobile) bacterial cells suspended in a fluid medium containing. NaCl or M=W12 brine, at various phaes of their growth cycIes. The number of suspended kcteria rangy-d born 106 to 109 cells ml-*. Tine amount of actinide associatd with the wspend~ cell fraction (cakzdated & mol cell-*) was very Iow: Th, 10-*2; U, 10-1s - 10-lS; - ~ Np, 1o-15- 10-19; Pu, 10-ls -10-21 ; and h, 10-1* - 10-*9 ; and it varied with the bacteihl - CUIture studied. l%e differe&es in the asswiation are amibuted to the extent of bioamxmdation and biosorption by the bacteria pH, the compo&on of the brine, and the speziation and bioavaiIability of the actinides.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/2439},
  journal      = {Radiochemeca Acta},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1998},

  month        = {12}

}

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