The structure and interaction of polymers affects secondary cell wall banding patterns in Arabidopsis

Pfaff, Sarah A.; Wagner, Edward R.; Cosgrove, Daniel J.

doi:10.1093/plcell/koae233

The structure and interaction of polymers affects secondary cell wall banding patterns in Arabidopsis

Journal Article · Tue Aug 20 00:00:00 EDT 2024 · The Plant Cell

DOI:https://doi.org/10.1093/plcell/koae233· OSTI ID:2455033

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Abstract

Xylem tracheary elements (TEs) synthesize patterned secondary cell walls (SCWs) to reinforce against the negative pressure of water transport. VASCULAR-RELATED NAC-DOMAIN 7 (VND7) induces differentiation, accompanied by cellulose, xylan, and lignin deposition into banded domains. To investigate the effect of polymer biosynthesis mutations on SCW patterning, we developed a method to induce tracheary element transdifferentiation of isolated protoplasts, by transient transformation with VND7. Our data showed that proper xylan elongation is necessary for distinct cellulose bands, cellulose–xylan interactions are essential for coincident polymer patterns, and cellulose deposition is needed to override the intracellular organization that yields unique xylan patterns. These data indicate that a properly assembled cell wall network acts as a scaffold to direct polymer deposition into distinctly banded domains. We describe the transdifferentiation of protoplasts into TEs, providing an avenue to study patterned SCW biosynthesis in a tissue-free environment and in various mutant backgrounds.

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Research Organization:: The Pennsylvania State University, University Park, PA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001090

OSTI ID:: 2455033

Alternate ID(s):: OSTI ID: 2438629
OSTI ID: 2459319

Journal Information:: The Plant Cell, Journal Name: The Plant Cell Journal Issue: 10 Vol. 36; ISSN 1040-4651

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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