Mouse models of Ebola virus tolerance and lethality: characterization of CD-1 mice infected with wild-type, guinea pig-adapted, or mouse-adapted virus

Spengler, Jessica R.; Welch, Stephen R.; Ritter, Jana M.; Harmon, Jessica R.; Coleman-McCray, JoAnn D.; Genzer, Sarah C.; Seixas, Josilene N.; Scholte, Florine E.M.; Davies, Katherine A.; Bradfute, Steven B.; Montgomery, Joel M.; Spiropoulou, Christina F.

doi:10.1016/j.antiviral.2022.105496

Mouse models of Ebola virus tolerance and lethality: characterization of CD-1 mice infected with wild-type, guinea pig-adapted, or mouse-adapted virus

Journal Article · Thu Dec 22 00:00:00 EST 2022 · Antiviral Research

DOI:https://doi.org/10.1016/j.antiviral.2022.105496· OSTI ID:2425745

^[1]; Welch, Stephen R. ^[1]; Ritter, Jana M. ^[1]; Harmon, Jessica R. ^[1]; Coleman-McCray, JoAnn D. ^[1]; Genzer, Sarah C. ^[1]; Seixas, Josilene N. ^[1]; Scholte, Florine E.M. ^[1]; Davies, Katherine A. ^[1]; Bradfute, Steven B. ^[2]; Montgomery, Joel M. ^[1]; Spiropoulou, Christina F. ^[1]

Centers for Disease Control and Prevention (CDC), Atlanta, GA (United States)
University of New Mexico Health Sciences Center, Albuquerque, NM (United States)

Development of lethal models of Ebola virus disease has been achieved by the serial passage of virus isolates from human cases in mice and guinea pigs. Use of mice infected with non-adapted virus has been limited due to the absence of overt clinical disease. In recent years, newly recognized sequelae identified in human cases has highlighted the importance of continued investigations of non-lethal infection both in humans and animal models. Here, we revisit the use of rodent-adapted and non-adapted Ebola virus (EBOV) in mice to investigate infection tolerance and future utility of these models in pathogenesis and therapeutic intervention studies. We found that like non-adapted wild-type EBOV, guinea pig-adapted EBOV resulted in widespread tissue infection, variably associated with tissue pathology, and alterations in clinical and immunological analytes in the absence of overt disease. Notably, infection with either non-lethal variant did not greatly differ from lethal mouse-adapted EBOV until near the time end-point criteria are reached in these mice. Furthermore, these data support future investigations of pathogenesis, convalescence, and sequelae in mouse models of virus tolerance.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 2425745

Alternate ID(s):: OSTI ID: 1960853

Journal Information:: Antiviral Research, Journal Name: Antiviral Research Journal Issue: C Vol. 210; ISSN 0166-3542

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Mouse models of Ebola virus tolerance and lethality: characterization of CD-1 mice infected with wild-type, guinea pig-adapted, or mouse-adapted virus

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