HPTLC Analysis and Chemical Composition of Selected Melaleuca Essential Oils

Vázquez, Aimé; Tabanca, Nurhayat; Kendra, Paul E.

doi:10.3390/molecules28093925

HPTLC Analysis and Chemical Composition of Selected Melaleuca Essential Oils

Journal Article · Sat May 06 00:00:00 EDT 2023 · Molecules

DOI:https://doi.org/10.3390/molecules28093925· OSTI ID:2425699

Vázquez, Aimé ^[1]; ^[1]; ^[1]

United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Miami, FL (United States)

Tea tree oil (TTO) is a volatile essential oil obtained by distillation, mainly from the Australian native plant Melaleuca alternifolia (Maiden & Betche) Cheel (Myrtaceae). In this study, a comparative analysis of the chemical constituents of seven tea tree oils (M. alternifolia) and four other Melaleuca spp. oils (M. cajuputi, (M_Ca), two chemotypes of M. quinquenervia, (M_Ne and M_Ni), and M. ericifolia (M_Ro)) was carried out using gas chromatography–mass spectrometry (GC-MS) and high-performance thin-layer chromatography (HPTLC). Among the seven TTOs, terpinen-4-ol (37.66–44.28%), γ-terpinene (16.42–20.75%), α-terpinene (3.47–12.62%), α-terpineol (3.11–4.66%), and terpinolene (2.75–4.19%) were the most abundant compounds. On the other hand, the most abundant compounds of the other Melaleuca oils varied, such as 1,8-cineole (64.63%) in M_Ca oil, (E)-nerolidol (48.40%) and linalool (33.30%) in M_Ne oil, 1,8-cineole (52.20%) in M_Ni oil, and linalool (38.19%) and 1,8-cineole (27.57%) in M_Ro oil. HPTLC fingerprinting of Melaleuca oils enabled the discrimination of TTO oils from other Melaleuca spp. oils. Variation was observed in the profile of the R_f values among EOs. The present study shows that HPTLC is one of the best ways to identify and evaluate the quality control in authenticating TTOs, other Melaleuca EOs, or EOs from other species within the Myrtaceae.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 2425699

Journal Information:: Molecules, Journal Name: Molecules Journal Issue: 9 Vol. 28; ISSN 1420-3049

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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HPTLC Analysis and Chemical Composition of Selected Melaleuca Essential Oils

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