Evaluation of Immunocompetent Mouse Models for Borrelia miyamotoi Infection

Armstrong, Brittany A.; Brandt, Kevin S.; Goodrich, Irina; Gilmore, Robert D.; Rogovskyy, Artem S.

doi:10.1128/spectrum.04301-22

Evaluation of Immunocompetent Mouse Models for Borrelia miyamotoi Infection

Journal Article · Mon Jan 30 00:00:00 EST 2023 · Microbiology Spectrum

DOI:https://doi.org/10.1128/spectrum.04301-22· OSTI ID:2425667

^[1]; Brandt, Kevin S. ^[1]; Goodrich, Irina ^[1]; ^[1]; Rogovskyy, Artem S. ^[2]

Centers for Disease Control and Prevention, Fort Collins, CO (United States)
Texas A & M University, College Station, TX (United States)

Borrelia miyamotoi is a relapsing fever spirochete that is harbored by Ixodes spp. ticks and is virtually uncharacterized, compared to other relapsing fever Borrelia vectored by Ornithodoros spp. ticks. There is not an immunocompetent mouse model for studying B. miyamotoi infection in vivo or for transmission in the vector-host cycle. Our goal was to evaluate B. miyamotoi infections in multiple mouse breeds/strains as a prelude to the ascertainment of the best experimental infection model. Two B. miyamotoi strains, namely, LB-2001 and CT13-2396, as well as three mouse models, namely, CD-1, C3H/HeJ, and BALB/c, were evaluated. We were unable to observe B. miyamotoi LB-2001 spirochetes in the blood via darkfield microscopy or to detect DNA via real-time PCR post needle inoculation in the CD-1 and C3H/HeJ mice. However, LB-2001 DNA was detected via real-time PCR in the blood of the BALB/c mice after needle inoculation, although spirochetes were not observed via microscopy. CD-1, C3H/HeJ, and BALB/c mice generated an antibody response to B. miyamotoi LB-2001 following needle inoculation, but established infections were not detected, and the I. scapularis larvae failed to acquire spirochetes from the exposed CD-1 mice. In contrast, B. miyamotoi CT13-2396 was visualized in the blood of the CD-1 and C3H/HeJ mice via darkfield microscopy and detected by real-time PCR post needle inoculation. Both mouse strains seroconverted. However, no established infection was detected in the mouse organs, and the I. scapularis larvae failed to acquire Borrelia after feeding on CT13-2396 exposed CD-1 or C3H/HeJ mice. Furthermore, these findings underscore the challenges in establishing an experimental B. miyamotoi infection model in immunocompetent laboratory mice.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 2425667

Journal Information:: Microbiology Spectrum, Journal Name: Microbiology Spectrum Journal Issue: 2 Vol. 11; ISSN 2165-0497

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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