Perivascular network segmentations derived from high-field MRI and their implications for perivascular and parenchymal mass transport in the rat brain

Rey, Julian A.; Farid, Uzair M.; Najjoum, Christopher M.; Brown, Alec; Magdoom, Kulam Najmudeen; Mareci, Thomas H.; Sarntinoranont, Malisa

doi:10.1038/s41598-023-34850-0

Perivascular network segmentations derived from high-field MRI and their implications for perivascular and parenchymal mass transport in the rat brain

Journal Article · Tue Jun 06 04:00:00 EDT 2023 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-023-34850-0· OSTI ID:2424560

Rey, Julian A. ^[1]; Farid, Uzair M. ^[1]; Najjoum, Christopher M. ^[1]; Brown, Alec ^[1]; Magdoom, Kulam Najmudeen ^[1]; Mareci, Thomas H. ^[1]; Sarntinoranont, Malisa ^[1]

University of Florida, Gainesville, FL (United States)

A custom segmentation workflow was applied to ex vivo high-field MR images of rat brains acquired following in vivo intraventricular contrast agent infusion to generate maps of the perivascular spaces (PVS). The resulting perivascular network segmentations enabled analysis of perivascular connections to the ventricles, parenchymal solute clearance, and dispersive solute transport within PVS. Numerous perivascular connections between the brain surface and the ventricles suggest the ventricles integrate into a PVS-mediated clearance system and raise the possibility of cerebrospinal fluid (CSF) return from the subarachnoid space to the ventricles via PVS. Assuming rapid solute exchange between the PVS and CSF spaces primarily by advection, the extensive perivascular network decreased the mean clearance distance from parenchyma to the nearest CSF compartment resulting in an over 21-fold reduction in the estimated diffusive clearance time scale, irrespective of solute diffusivity. This corresponds to an estimated diffusive clearance time scale under 10 min for amyloid-beta which suggests that the widespread distribution of PVS may render diffusion an effective parenchymal clearance mechanism. Additional analysis of oscillatory solute dispersion within PVS indicates that advection rather than dispersion is likely the primary transport mechanism for dissolved compounds greater than 66 kDa in the long (> 2 mm) perivascular segments identified here, although dispersion may be significant for smaller compounds in shorter perivascular segments.

View Accepted Manuscript (DOE)

Research Organization:: University of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE

OSTI ID:: 2424560

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 13; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Perivascular network segmentations derived from high-field MRI and their implications for perivascular and parenchymal mass transport in the rat brain

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