Dioxin TECs in lake trout derived with liver cell lines from rainbow trout and rat
- Univ. of Waterloo, Ontario (Canada). Dept. of Biology
The RTL-WL rainbow trout liver and H4IIE rat hepatoma cell lines were exposed to organic solvent extracts from livers of lake trout collected from two Lake Superior sites (Jackfish Bay -- a pulp mill site and Black Bay -- a reference site) and one Lake Ontario site (Eastern Basin -- a PCB-contaminated site). These two bioassays measure the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxic equivalent concentrations (TECs) of the residues as indicated by their relative ability to induce 7-ethoxyresorufin-o-deethylase (EROD) activity. This estimates the burden of compounds that could act through the Ah receptor and are potentially toxic, Subsamples of liver were also analyzed for hepatic EROD activity. For Jackfish Bay extracts, the H4IIE mean TEC was found to be significantly higher than that derived using RTL-WL, indicating a more potent contaminant mixture to this mammalian model. No such difference was seen between mean TECs derived for Lake Ontario extracts. The reference site, Black Bay, had TECs significantly lower than those from the other two sites. A significant positive correlation between TECs derived with H4IIE vs. RTL-WL was seen for Jackfish Bay, but not Lake Ontario. Lake Ontario extracts had a significantly higher mean TEC than the mean TEC from Jackfish Bay regardless of the cell line used. Hepatic EROD activities of liver subsamples from Jackfish Bay were found to be significantly higher than subsamples from Black Bay, while Lake Ontario subsamples were variable. No significant correlation was found between hepatic EROD activity and TEC values. The RTL-WL cell line bioassay was found to have excellent utility for rapid TEC determination and should be generally suitable for estimating the toxic potency of environmental samples.
- OSTI ID:
- 242322
- Report Number(s):
- CONF-9511137--; ISBN 1-880611-03-1
- Country of Publication:
- United States
- Language:
- English
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