Regulation of hair cell and stomatal size by a hair cell-specific peroxidase in the grass Brachypodium distachyon

Nunes, Tiago D.G.; Berg, Lea S.; Slawinska, Magdalena W.; Zhang, Dan; Redt, Leonie; Sibout, Richard; Vogel, John P.; Laudencia-Chingcuanco, Debbie; Jesenofsky, Barbara; Lindner, Heike; Raissig, Michael T.

doi:10.1016/j.cub.2023.03.089

Regulation of hair cell and stomatal size by a hair cell-specific peroxidase in the grass Brachypodium distachyon

Journal Article · Fri Apr 21 00:00:00 EDT 2023 · Current Biology

DOI:https://doi.org/10.1016/j.cub.2023.03.089· OSTI ID:2422828

^[1]; ^[2]; ^[3]; ^[3]; Redt, Leonie ^[3]; Sibout, Richard ^[4]; Vogel, John P. ^[5]; Laudencia-Chingcuanco, Debbie ^[6]; Jesenofsky, Barbara ^[3]; ^[7]; ^[7]

Heidelberg Univ. (Germany); Univ. of Helsinki (Finland)
Univ. of Bern (Switzerland)
Heidelberg Univ. (Germany)
French National Research Institute for Agriculture, Food and Environment (INRAE), Nantes (France). Biopolymères Interactions Assemblages (BIA) UR1268
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
US Dept. of Agriculture (USDA), Albany, CA (United States). Western Regional Research Center (WRRC)
Heidelberg Univ. (Germany); Univ. of Bern (Switzerland)

The leaf epidermis is the outermost cell layer forming the interface between plants and the atmosphere that must both provide a robust barrier against (a)biotic stressors and facilitate carbon dioxide uptake and leaf transpiration. To achieve these opposing requirements, the plant epidermis developed a wide range of specialized cell types such as stomata and hair cells. While factors forming these individual cell types are known, it is poorly understood how their number and size is coordinated. Here, in this study, we identified a role for BdPRX76/BdPOX, a class III peroxidase, in regulating hair cell and stomatal size in the model grass Brachypodium distachyon. In bdpox mutants prickle hair cells were smaller and stomata were longer. Because stomatal density remained unchanged, the negative correlation between stomatal size and density was disrupted in bdpox and resulted in higher stomatal conductance and lower intrinsic water-use efficiency. BdPOX was exclusively expressed in hair cells suggesting that BdPOX cell autonomously promotes hair cell size and indirectly restricts stomatal length. Cell wall autofluorescence and lignin stainings indicated a role for BdPOX in lignification or crosslinking of related phenolic compounds at the hair cell base. Ectopic expression of BdPOX in the stomatal lineage increased phenolic autofluorescence in guard cell walls and restricted stomatal elongation in bdpox. Together, we highlight a developmental interplay between hair cells and stomata that optimizes epidermal functionality. We propose that cell-type-specific changes disrupt this interplay and lead to compensatory developmental defects in other epidermal cell types.

View Accepted Manuscript (DOE)

Research Organization:: USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

Sponsoring Organization:: German Research Foundation (DFG); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2422828

Journal Information:: Current Biology, Journal Name: Current Biology Journal Issue: 9 Vol. 33; ISSN 0960-9822

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Biochemistry & Molecular Biology
Brachypodium distachyon
Cell Biology
Life Sciences & Biomedicine - Other Topics
cell size
class III peroxidase
gas exchange
grasses
lignin
prickle hair cells
stomata

Regulation of hair cell and stomatal size by a hair cell-specific peroxidase in the grass Brachypodium distachyon

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