Trichoderma harzianum transcriptome in response to the nematode Pratylenchus brachyurus

de Oliveira, Camilla Martins; Oshiquiri, Letícia Harumi; Almeida, Nayane Oliveira; Steindorf, Andrei Stecca; da Rocha, Mara Rúbia; Georg, Raphaela Castro; Ulhoa, Cirano José

doi:10.1016/j.biocontrol.2023.105245

Trichoderma harzianum transcriptome in response to the nematode Pratylenchus brachyurus

Journal Article · Tue May 02 00:00:00 EDT 2023 · Biological Control

DOI:https://doi.org/10.1016/j.biocontrol.2023.105245· OSTI ID:2422798

de Oliveira, Camilla Martins ^[1]; Oshiquiri, Letícia Harumi ^[2]; Almeida, Nayane Oliveira ^[1]; Steindorf, Andrei Stecca ^[3]; da Rocha, Mara Rúbia ^[1]; Georg, Raphaela Castro ^[1]; Ulhoa, Cirano José ^[1]

Federal University of Goiás (UFG) (Brazil)
University of Sao Paulo (Brazil)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

The root-lesion nematode Pratylenchus brachyurus causes extensive damage in several crops of economic importance. Fungi of Trichoderma genus have been highlighted as biopesticide agents in the control of several plant diseases. Although it is already widely used in agriculture, there are few studies, especially at the molecular level, that evaluate T. harzianum in the control of P. brachyurus. The aim of the present study was to investigate how interaction with the nematode P. brachyurus influences gene expression of T. harzianum, by using RNA-Seq analysis. Of the 13,932 predicted genes in the T. harzianum genome, 2,922 (21%) were differentially expressed in the presence of P. brachyurus, in relation to the absence of the nematode. Among the differentially expressed genes, we found genes encoding Carbohydrate Active EnZymes (CAZy), MEROPS peptidases, and proteins related to secondary metabolite synthesis. 118 pathways were identified as related to the biosynthesis of secondary metabolites. Additionally, the analysis identified 136 metabolic pathways related to these differentially expressed genes, among which we highlight: aminobenzoate degradation, xenobiotic metabolism by cytochrome P450, and sesquiterpenoid and triterpenoid biosynthesis. Our results contribute to a better understanding of the response of T. harzianum to the nematode P. bracyurus, the potential for biocontrol by this fungus.

View Accepted Manuscript (DOE)

Research Organization:: USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2422798

Journal Information:: Biological Control, Journal Name: Biological Control Journal Issue: C Vol. 183; ISSN 1049-9644

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Trichoderma harzianum transcriptome in response to the nematode Pratylenchus brachyurus

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