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Cell-Free Protein Synthesis of Particulate Methane Monooxygenase into Nanodiscs

Journal Article · · ACS Synthetic Biology
 [1];  [2];  [3];  [4]
  1. Department of Molecular Biosciences and of Chemistry, Northwestern University, Evanston, Illinois60208, United States; OSTI
  2. Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois60208, United States
  3. Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois60208, United States; Center for Synthetic Biology, Northwestern University, Evanston, Illinois60208, United States
  4. Department of Molecular Biosciences and of Chemistry, Northwestern University, Evanston, Illinois60208, United States; Center for Synthetic Biology, Northwestern University, Evanston, Illinois60208, United States
Not provided.
Research Organization:
Northwestern Univ., Evanston, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
DOE Contract Number:
SC0018249
OSTI ID:
2422508
Journal Information:
ACS Synthetic Biology, Journal Name: ACS Synthetic Biology Journal Issue: 12 Vol. 11; ISSN 2161-5063
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English

References (55)

Membrane protein production in Escherichia coli cell-free lysates journal May 2015
Rising methane: A new climate challenge journal June 2019
Methanotrophs and copper journal July 2010
Cell-free synthesis of membrane subunits of ATP synthase in phospholipid bicelles: NMR shows subunit a fold similar to the protein in the cell membrane : Cell-Free Synthesis of Membrane Subunits of ATP Synthase journal January 2012
Consequences of the Overexpression of a Eukaryotic Membrane Protein, the Human KDEL Receptor, in Escherichia coli journal April 2011
Cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates journal January 2019
Overcoming barriers to membrane protein structure determination journal April 2011
Efficient and scalable method for scaling up cell free protein synthesis in batch mode journal January 2005
Regional trends and drivers of the global methane budget journal October 2021
Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer journal March 2022
Rethinking biological activation of methane and conversion to liquid fuels journal April 2014
High-Energy-Resolution Fluorescence-Detected X-ray Absorption of the Q Intermediate of Soluble Methane Monooxygenase journal November 2017
Cell‐free synthesis of functional aquaporin Z in synthetic liposomes journal June 2009
Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles journal April 2021
Cell-Free Expression and Assembly of ATP Synthase journal October 2011
High-Resolution XFEL Structure of the Soluble Methane Monooxygenase Hydroxylase Complex with its Regulatory Component at Ambient Temperature in Two Oxidation States journal July 2020
Ecology and Diversity of Methylotrophic Organisms book January 1980
Hydrophobic supplements in cell-free systems: Designing artificial environments for membrane proteins journal March 2014
Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing journal March 2019
Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane journal January 2005
A tale of two methane monooxygenases journal November 2016
High-throughput preparation methods of crude extract for robust cell-free protein synthesis journal March 2015
Cell-free gene expression: an expanded repertoire of applications journal November 2019
Particulate methane monooxygenase genes in methanotrophs. journal January 1995
Coordination of the Copper Centers in Particulate Methane Monooxygenase: Comparison between Methanotrophs and Characterization of the CuC Site by EPR and ENDOR Spectroscopies journal September 2021
Cell-Free Synthetic Biology: Engineering Beyond the Cell journal October 2016
An integrated cell-free metabolic platform for protein production and synthetic biology journal October 2008
Cell-free protein synthesis: Applications come of age journal September 2012
Membrane protein assembly into Nanodiscs journal October 2009
Current strategies for protein production and purification enabling membrane protein structural biology journal December 2016
Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids journal March 2018
Cell‐free protein synthesis enables high yielding synthesis of an active multicopper oxidase journal September 2015
Particulate methane monooxygenase contains only mononuclear copper centers journal May 2019
Shaping Escherichia coli for recombinant membrane protein production journal June 2018
Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase journal June 2019
Cell-free expression as an emerging technique for the large scale production of integral membrane protein journal September 2006
Methanobactins: Maintaining copper homeostasis in methanotrophs and beyond journal January 2018
Biochemistry of aerobic biological methane oxidation journal January 2021
Methane as a Resource: Can the Methanotrophs Add Value? journal March 2015
Oxidation of methane by a biological dicopper centre journal April 2010
The CopC Family: Structural and Bioinformatic Insights into a Diverse Group of Periplasmic Copper Binding Proteins journal April 2016
New tools for automated high-resolution cryo-EM structure determination in RELION-3 journal November 2018
In-Situ Observation of Membrane Protein Folding during Cell-Free Expression journal March 2016
Biological conversion of methane to methanol through genetic reassembly of native catalytic domains journal April 2019
Soluble Methane Monooxygenase journal June 2019
Enzymatic Oxidation of Methane journal March 2015
Nanodiscs: A Controlled Bilayer Surface for the Study of Membrane Proteins journal May 2018
From micelles to bicelles: Effect of the membrane on particulate methane monooxygenase activity journal May 2018
Crystal Structure and Characterization of Particulate Methane Monooxygenase from Methylocystis species Strain M journal November 2011
Structural Studies of the Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase and Regulatory Component Complex Reveal a Transient Substrate Tunnel journal July 2020
Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes journal June 2014
Effects of Zinc on Particulate Methane Monooxygenase Activity and Structure journal June 2014
Lipid–protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: Comparison with detergent micelles, bicelles and liposomes journal March 2012
A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases journal December 2017
Mimicking theEscherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis journal January 2004

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