A Polymer Canvas with the Stiffness of the Bone Matrix to Study and Control Mesenchymal Stem Cell Response

Zanut, Alessandra; Li, Rui; Deng, Ru; Liu, Xiangyu; Rejhon, Martin; Chen, Weiqiang; Weck, Marcus; de Peppo, Giuseppe Maria; Riedo, Elisa

doi:10.1002/adhm.202201503

A Polymer Canvas with the Stiffness of the Bone Matrix to Study and Control Mesenchymal Stem Cell Response

Journal Article · Sat Dec 24 00:00:00 EST 2022 · Advanced Healthcare Materials

DOI:https://doi.org/10.1002/adhm.202201503· OSTI ID:2420876

Zanut, Alessandra ^[1]; Li, Rui ^[2]; Deng, Ru ^[3]; Liu, Xiangyu ^[2]; Rejhon, Martin ^[2]; Chen, Weiqiang ^[2]; Weck, Marcus ^[3]; ^[4]; ^[2]

New York Univ. (NYU), Brooklyn, NY (United States); OSTI
New York Univ. (NYU), Brooklyn, NY (United States)
New York Univ. (NYU), NY (United States)
New York Univ. (NYU), Brooklyn, NY (United States); New York Stem Cell Foundation Research Institute, New York, NY (United States); Mirimus, Inc., Brooklyn, NY (United States)

Reproducing in vitro the complex multiscale physical features of human tissues creates novel biomedical opportunities and fundamental understanding of cell–environment interfaces and interactions. While stiffness has been recognized as a key driver of cell behavior, systematic studies on the role of stiffness have been limited to values in the KPa–MPa range, significantly below the stiffness of bone. Here, in this work, a platform enabling the tuning of the stiffness of a biocompatible polymeric interface up to values characteristic of human bone is reported, which are in the GPa range, by using extremely thin polymer films on glass and cross-linking the films using ultraviolet (UV) light irradiation. It is shown that a higher stiffness is related to better adhesion, proliferation, and osteogenic differentiation, and that it is possible to switch on/off cell attachment and growth by solely tuning the stiffness of the interface, without any surface chemistry or topography modification. Since the stiffness is tuned directly by UV irradiation, this platform is ideal for rapid and simple fabrication of stiffness patterns and gradients, thus representing an innovative tool for combinatorial studies of the synergistic effect of tissue environmental cues on cell behavior, and creates new opportunities for next-generation biosensors, single-cell patterning, and lab-on-a-chip devices.

View Accepted Manuscript (DOE)

Research Organization:: New York Univ. (NYU), NY (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0018924

OSTI ID:: 2420876

Alternate ID(s):: OSTI ID: 1983369

Journal Information:: Advanced Healthcare Materials, Journal Name: Advanced Healthcare Materials Journal Issue: 10 Vol. 12; ISSN 2192-2640

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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60 APPLIED LIFE SCIENCES
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biomimicry
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A Polymer Canvas with the Stiffness of the Bone Matrix to Study and Control Mesenchymal Stem Cell Response

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