Highly Symmetric, Self-Assembling 3D DNA Crystals with Cubic and Trigonal Lattices

Lu, Brandon; Vecchioni, Simon; Ohayon, Yoel P.; Woloszyn, Karol; Markus, Tiffany; Mao, Chengde; Seeman, Nadrian C.; Canary, James W.; Sha, Ruojie

doi:10.1002/smll.202205830

Highly Symmetric, Self-Assembling 3D DNA Crystals with Cubic and Trigonal Lattices

Journal Article · Sun Nov 20 23:00:00 EST 2022 · Small

DOI:https://doi.org/10.1002/smll.202205830· OSTI ID:2419795

Lu, Brandon ^[1]; Vecchioni, Simon ^[2]; Ohayon, Yoel P. ^[2]; Woloszyn, Karol ^[2]; Markus, Tiffany ^[2]; Mao, Chengde ^[3]; Seeman, Nadrian C. ^[2]; Canary, James W. ^[2]; ^[2]

New York Univ. (NYU), NY (United States); OSTI
New York Univ. (NYU), NY (United States)
Purdue Univ., West Lafayette, IN (United States)

The rational design of nanoscopic DNA tiles has yielded highly ordered crystalline matter in 2D and 3D. The most well-studied 3D tile is the DNA tensegrity triangle, which is known to self-assemble into macroscopic crystals. However, contemporary rational design parameters for 3D DNA crystals nearly universally invoke integer numbers of DNA helical turns and Watson–Crick (WC) base pairs. Here, in this study, 24-bp edges are substituted into a previously 21-bp (two helical turns of DNA) tensegrity triangle motif to explore whether such unconventional motif can self-assemble into 3D crystals. The use of noncanonical base pairs in the sticky ends results in a cubic arrangement of tensegrity triangles with exceedingly high symmetry, assembling a lattice from winding helical axes and diamond-like tessellation patterns. Reverting this motif to sticky ends with Watson–Crick pairs results in a trigonal hexagonal arrangement, replicating this diamond arrangement in a hexagonal context. These results showcase that the authors can generate unexpected, highly complex, pathways for materials design by testing modifications to 3D tiles without prior knowledge of the ensuing symmetry. This study expands the rational design toolbox for DNA nanotechnology; and it further illustrates the existence of yet-unexplored arrangements of crystalline soft matter.

View Accepted Manuscript (DOE)

Research Organization:: New York Univ. (NYU), NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC); US Department of the Navy, Office of Naval Research (ONR); National Science Foundation (NSF)

Grant/Contract Number:: SC0007991

OSTI ID:: 2419795

Alternate ID(s):: OSTI ID: 1899185

Journal Information:: Small, Journal Name: Small Journal Issue: 3 Vol. 19; ISSN 1613-6810

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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