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Semisynthetic maturation of [FeFe]-hydrogenase using [Fe2 (μ-SH)2 (CN)2 (CO)4 ]2-: key roles for HydF and GTP

Journal Article · · ChemComm
DOI:https://doi.org/10.1039/d3cc02169f· OSTI ID:2419700
 [1];  [2];  [2];  [2];  [2];  [2];  [2];  [2]
  1. Montana State Univ., Bozeman, MT (United States); OSTI
  2. Montana State Univ., Bozeman, MT (United States)
+ Show Author Affiliations

The [FeFe]-hydrogenase can be matured using a defined semisynthetic approach in which the synthetic complex [2Fe]E bypasses the need for HydE and HydG, and glycine cleavage system components synthesize DTMA on [2Fe]E while it is bound to HydF.

Research Organization:
Montana State Univ., Bozeman, MT (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
SC0005404
OSTI ID:
2419700
Alternate ID(s):
OSTI ID: 1987584
Journal Information:
ChemComm, Journal Name: ChemComm Journal Issue: 58 Vol. 59; ISSN CHCOFS; ISSN 1359-7345
Publisher:
Royal Society of ChemistryCopyright Statement
Country of Publication:
United States
Language:
English

References (39)

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The [4Fe‐4S]‐Cluster of HydF is not Required for the Binding and Transfer of the Diiron Site of [FeFe]‐Hydrogenases journal May 2023
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[FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation journal June 2015
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The [FeFe]-hydrogenase maturase HydF from Clostridium acetobutylicum contains a CO and CN − ligated iron cofactor journal December 2009
Hydrogenase Enzymes and Their Synthetic Models: The Role of Metal Hydrides journal June 2016
Biosynthesis of the [FeFe] Hydrogenase H Cluster: A Central Role for the Radical SAM Enzyme HydG journal November 2015
Biochemical and Kinetic Characterization of Radical S -Adenosyl- l -methionine Enzyme HydG journal November 2013
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Activation of HydA ΔEFG Requires a Preformed [4Fe-4S] Cluster journal July 2009
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[FeFe]-Hydrogenase Maturation: HydG-Catalyzed Synthesis of Carbon Monoxide journal July 2010
Reversible H Atom Abstraction Catalyzed by the Radical S -Adenosylmethionine Enzyme HydG journal September 2014
Radical SAM Enzyme HydE Generates Adenosylated Fe(I) Intermediates En Route to the [FeFe]-Hydrogenase Catalytic H-Cluster journal May 2020
Crystal Structure of the [FeFe]-Hydrogenase Maturase HydE Bound to Complex-B journal May 2021
Organometallic Fe2(μ-SH)2(CO)4(CN)2 Cluster Allows the Biosynthesis of the [FeFe]-Hydrogenase with Only the HydF Maturase journal January 2022
The Radical SAM Enzyme HydG Requires Cysteine and a Dangler Iron for Generating an Organometallic Precursor to the [FeFe]-Hydrogenase H-Cluster journal January 2016
Biomimetic assembly and activation of [FeFe]-hydrogenases journal June 2013
A [4Fe–4S]-Fe(CO)(CN)-l-cysteine intermediate is the first organometallic precursor in [FeFe] hydrogenase H-cluster bioassembly journal April 2018
Compositional and structural insights into the nature of the H-cluster precursor on HydF journal January 2018
Serine is the molecular source of the NH(CH 2 ) 2 bridgehead moiety of the in vitro assembled [FeFe] hydrogenase H-cluster journal January 2020
Biosynthesis of the catalytic H-cluster of [FeFe] hydrogenase: the roles of the Fe–S maturase proteins HydE, HydF, and HydG journal January 2020
HydG, the “dangler” iron, and catalytic production of free CO and CN − : implications for [FeFe]-hydrogenase maturation journal January 2021
Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii journal February 2005
Synthesis of the 2Fe subcluster of the [FeFe]-hydrogenase H cluster on the HydF scaffold journal May 2010
X-ray crystallographic and EPR spectroscopic analysis of HydG, a maturase in [FeFe]-hydrogenase H-cluster assembly journal January 2015
CO and CN − syntheses by [FeFe]-hydrogenase maturase HydG are catalytically differentiated events journal December 2015
Biochemical Analysis of the Interactions between the Proteins Involved in the [FeFe]-Hydrogenase Maturation Process journal August 2012
Discovery of Two Novel Radical S -Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase journal April 2004
The [Fe-Fe]-Hydrogenase Maturation Protein HydF from Thermotoga maritima Is a GTPase with an Iron-Sulfur Cluster journal November 2005
Radical S -Adenosyl-l-methionine Chemistry in the Synthesis of Hydrogenase and Nitrogenase Metal Cofactors journal December 2014
The HydG Enzyme Generates an Fe(CO)2(CN) Synthon in Assembly of the FeFe Hydrogenase H-Cluster journal January 2014
Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System journal March 2006
Tyrosine, Cysteine, and S-Adenosyl Methionine Stimulate In Vitro [FeFe] Hydrogenase Activation journal October 2009
Overview of the Maturation Machinery of the H-Cluster of [FeFe]-Hydrogenases with a Focus on HydF journal October 2018

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY