Coupling AFEX and steam-exploded sugarcane residue pellets with a room temperature CIIII-activation step lowered enzyme dosage requirements for sugar conversion

Mokomele, Thapelo; da Costa Sousa, Leonardo; Colbert, Abby; Dale, Bruce E.; Görgens, Johann F.; Balan, Venkatesh

doi:10.1016/j.cej.2022.137117

Coupling AFEX and steam-exploded sugarcane residue pellets with a room temperature CIIII-activation step lowered enzyme dosage requirements for sugar conversion

Journal Article · Mon May 23 00:00:00 EDT 2022 · Chemical Engineering Journal

DOI:https://doi.org/10.1016/j.cej.2022.137117· OSTI ID:2418898

Mokomele, Thapelo ^[1]; da Costa Sousa, Leonardo ^[2]; Colbert, Abby ^[3]; Dale, Bruce E. ^[4]; Görgens, Johann F. ^[5]; ^[6]

University of Stellenbosch (South Africa); Michigan State Univ., East Lansing, MI (United States). Biomass Conversion Research Laboratory; OSTI
Michigan State Univ., East Lansing, MI (United States). Biomass Conversion Research Laboratory
Univ. of Houston, Sugarland, TX (United States)
Michigan State Univ., East Lansing, MI (United States). Biomass Conversion Research Laboratory; Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center (GLBRC)
University of Stellenbosch (South Africa)
Univ. of Houston, Sugarland, TX (United States); Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center (GLBRC)

In this study, the potential integration of steam explosion (StEx) and ammonia fiber expansion (AFEX) with existing sugar/ethanol mills to form decentralized pre-processing depots was explored. Both StEx and AFEX pretreatment facilitated the production of sugarcane bagasse (SCB) and cane leaf matter (CLM) pellets with significantly higher bulk density, mechanical durability, and hydrophobicity relative to their untreated biomass pellet controls. However, ethanol production from standalone StEx and AFEX-treated SCB and CLM pellets required enzyme dosages greater than 21 mg/g glucan to achieve enzymatic hydrolysis sugar yields of 75% and ethanol titres greater than 40 g.L^-1. Coupling AFEX-treated SCB or CLM pellets with a room temperature CIII_I-activation step using liquid ammonia lowered enzyme dosage requirements by more than 50% without affecting ethanol titers and production yields (greater than300 L per Mg residual dry matter raw dry biomass (RDM)). In contrast, treating StEx-treated pellets with CIIII-activation using liquid ammonia did not result in similar enzyme dosage reductions, due to pseudo-lignin formation, leading to enzyme deactivation and/or lignin blockage that retarded enzymatic hydrolysis at low enzyme dosages. A gross energy conversion assessment revealed that low enzyme dosage (3-4 mg enzyme/g RDM) ethanol and electricity co-production from AFEX and CIII_I-activated SCB and CLM can recover up to 73% of the energy in the untreated biomass, compared to 54% recovered by StEx and CIII_I-activation. The results from this work suggest that StEx or AFEX based pre-processing depots can produce dense and mechanically durable biomass pellets. The AFEX-treated pellets can be easily upgraded using a room temperature CIII_I-activation step at the biorefinery to significantly reduce bioconversion enzymes.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 2418898

Alternate ID(s):: OSTI ID: 1961453

Journal Information:: Chemical Engineering Journal, Journal Name: Chemical Engineering Journal Journal Issue: P2 Vol. 446; ISSN 1385-8947

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (55)

Investigation of biochemical biorefinery sizing and environmental sustainability impacts for conventional bale system and advanced uniform biomass logistics designs Argo, Andrew M.; Tan, Eric CD; Inman, Daniel Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 3 https://doi.org/10.1002/bbb.1391	journal	April 2013
All biomass is local: The cost, volume produced, and global warming impact of cellulosic biofuels depend strongly on logistics and local conditions Kim, Seungdo; Dale, Bruce E. Biofuels, Bioproducts and Biorefining, Vol. 9, Issue 4 https://doi.org/10.1002/bbb.1554	journal	April 2015
Strengths, challenges, and opportunities for hydrothermal pretreatment in lignocellulosic biorefineries Yang, Bin; Tao, Ling; Wyman, Charles E. Biofuels, Bioproducts and Biorefining, Vol. 12, Issue 1 https://doi.org/10.1002/bbb.1825	journal	October 2017
Time to Rethink Cellulosic Biofuels? Dale, Bruce Biofuels, Bioproducts and Biorefining, Vol. 12, Issue 1 https://doi.org/10.1002/bbb.1856	journal	January 2018
Using steam explosion or AFEX™ to produce animal feeds and biofuel feedstocks in a biorefinery based on sugarcane residues Mokomele, Thapelo; da Costa Sousa, Leonardo; Bals, Bryan Biofuels, Bioproducts and Biorefining, Vol. 12, Issue 6 https://doi.org/10.1002/bbb.1927	journal	August 2018
Economic evaluation and comparison of succinic acid and electricity co‐production from sugarcane bagasse and trash lignocelluloses in a biorefinery, using different pretreatment methods: dilute acid (H₂SO₄), alkaline (NaOH), organosolv, ammonia fibre expansion (AFEX™), steam explosion (STEX), and wet oxidation Nieder‐Heitmann, Mieke; Haigh, Kathleen; Louw, Janus Biofuels, Bioproducts and Biorefining, Vol. 14, Issue 1 https://doi.org/10.1002/bbb.2020	journal	May 2019
The challenge of enzyme cost in the production of lignocellulosic biofuels Klein-Marcuschamer, Daniel; Oleskowicz-Popiel, Piotr; Simmons, Blake A. Biotechnology and Bioengineering, Vol. 109, Issue 4 https://doi.org/10.1002/bit.24370	journal	November 2011
Enzymatic hydrolysis of pelletized AFEX™-treated corn stover at high solid loadings: Hydrolysis of Pelletized AFEX Corn Stover Bals, Bryan D.; Gunawan, Christa; Moore, Janette Biotechnology and Bioengineering, Vol. 111, Issue 2 https://doi.org/10.1002/bit.25022	journal	September 2013
Changes in various physical/chemical parameters of Pinus pinaster wood after steam explosion pretreatment Negro, M. J.; Manzanares, P.; Oliva, J. M. Biomass and Bioenergy, Vol. 25, Issue 3 https://doi.org/10.1016/S0961-9534(03)00017-5	journal	September 2003
Cost optimization of biofuel production – The impact of scale, integration, transport and supply chain configurations de Jong, Sierk; Hoefnagels, Ric; Wetterlund, Elisabeth Applied Energy, Vol. 195 https://doi.org/10.1016/j.apenergy.2017.03.109	journal	June 2017
Comparing alternative cellulosic biomass biorefining systems: Centralized versus distributed processing systems Kim, Seungdo; Dale, Bruce E. Biomass and Bioenergy, Vol. 74 https://doi.org/10.1016/j.biombioe.2015.01.018	journal	March 2015
Optimization of lignocellulosic biomass-to-biofuel supply chains with densification: Literature review Albashabsheh, Nibal T.; Heier Stamm, Jessica L. Biomass and Bioenergy, Vol. 144 https://doi.org/10.1016/j.biombioe.2020.105888	journal	January 2021
Mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion (AFEX) pretreated corn stover Gao, Dahai; Chundawat, Shishir P. S.; Krishnan, Chandraraj Bioresource Technology, Vol. 101, Issue 8, p. 2770-2781 https://doi.org/10.1016/j.biortech.2009.10.056	journal	April 2010
Evaluating the impact of ammonia fiber expansion (AFEX) pretreatment conditions on the cost of ethanol production Bals, Bryan; Wedding, Chris; Balan, Venkatesh Bioresource Technology, Vol. 102, Issue 2, p. 1277-1283 https://doi.org/10.1016/j.biortech.2010.08.058	journal	January 2011
Integrated versus stand-alone second generation ethanol production from sugarcane bagasse and trash Dias, Marina O. S.; Junqueira, Tassia L.; Cavalett, Otávio Bioresource Technology, Vol. 103, Issue 1 https://doi.org/10.1016/j.biortech.2011.09.120	journal	January 2012
Evaluation of electricity generation from lignin residue and biogas in cellulosic ethanol production Liu, Gang; Bao, Jie Bioresource Technology, Vol. 243 https://doi.org/10.1016/j.biortech.2017.07.022	journal	November 2017
Incorporating anaerobic co-digestion of steam exploded or ammonia fiber expansion pretreated sugarcane residues with manure into a sugarcane-based bioenergy-livestock nexus Mokomele, Thapelo; da Costa Sousa, Leonardo; Balan, Venkatesh Bioresource Technology, Vol. 272 https://doi.org/10.1016/j.biortech.2018.10.049	journal	January 2019
Better use of bioenergy: A critical review of co-pelletizing for biofuel manufacturing Cui, Xuyang; Yang, Junhong; Wang, Zeyu Carbon Capture Science & Technology, Vol. 1 https://doi.org/10.1016/j.ccst.2021.100005	journal	December 2021
Cellulosic ethanol: status and innovation Lynd, Lee R.; Liang, Xiaoyu; Biddy, Mary J. Current Opinion in Biotechnology, Vol. 45 https://doi.org/10.1016/j.copbio.2017.03.008	journal	June 2017
A new insight into sugarcane biorefineries with fossil fuel co-combustion: Techno-economic analysis and life cycle assessment Mandegari, Mohsen; Farzad, Somayeh; Görgens, Johann F. Energy Conversion and Management, Vol. 165 https://doi.org/10.1016/j.enconman.2018.03.057	journal	June 2018
Techno-economic comparison of process technologies for biochemical ethanol production from corn stover Kazi, Feroz Kabir; Fortman, Joshua A.; Anex, Robert P. Fuel, Vol. 89, p. S20-S28 https://doi.org/10.1016/j.fuel.2010.01.001	journal	November 2010
Evaluation of bagasse from different varieties of sugarcane by dilute acid pretreatment and enzymatic hydrolysis Benjamin, Y.; Cheng, H.; Görgens, J. F. Industrial Crops and Products, Vol. 51 https://doi.org/10.1016/j.indcrop.2013.08.067	journal	November 2013
Factors affecting wood, energy grass and straw pellet durability – A review Whittaker, Carly; Shield, Ian Renewable and Sustainable Energy Reviews, Vol. 71 https://doi.org/10.1016/j.rser.2016.12.119	journal	May 2017
Lignocellulosic biorefineries: The current state of challenges and strategies for efficient commercialization Usmani, Zeba; Sharma, Minaxi; Awasthi, Abhishek Kumar Renewable and Sustainable Energy Reviews, Vol. 148 https://doi.org/10.1016/j.rser.2021.111258	journal	September 2021
Year-Round Storage Operation of Three Major Agricultural Crop Residue Biomasses by Performing Dry Acid Pretreatment at Regional Collection Depots Han, Tao; Zhang, Bin; Li, Hongxing ACS Sustainable Chemistry & Engineering, Vol. 9, Issue 13 https://doi.org/10.1021/acssuschemeng.0c08739	journal	March 2021
Restructuring the Crystalline Cellulose Hydrogen Bond Network Enhances Its Depolymerization Rate Chundawat, Shishir P. S.; Bellesia, Giovanni; Uppugundla, Nirmal Journal of the American Chemical Society, Vol. 133, Issue 29 https://doi.org/10.1021/ja2011115	journal	July 2011
Cellulose III _I Crystal Structure and Hydrogen Bonding by Synchrotron X-ray and Neutron Fiber Diffraction Wada, Masahisa; Chanzy, Henri; Nishiyama, Yoshiharu Macromolecules, Vol. 37, Issue 23 https://doi.org/10.1021/ma0485585	journal	November 2004
X-ray Structure of Ammonia−Cellulose I: New Insights into the Conversion of Cellulose I to Cellulose III _I Wada, Masahisa; Nishiyama, Yoshiharu; Langan, Paul Macromolecules, Vol. 39, Issue 8 https://doi.org/10.1021/ma060228s	journal	April 2006
Lignin repolymerisation in spruce autohydrolysis pretreatment increases cellulase deactivation Pielhop, Thomas; Larrazábal, Gastón O.; Studer, Michael H. Green Chemistry, Vol. 17, Issue 6 https://doi.org/10.1039/C4GC02381A	journal	January 2015
Next-generation ammonia pretreatment enhances cellulosic biofuel production da Costa Sousa, Leonardo; Jin, Mingjie; Chundawat, Shishir P. S. Energy & Environmental Science, Vol. 9, Issue 4 https://doi.org/10.1039/C5EE03051J	journal	January 2016
Autohydrolysis pretreatment of softwood – enhancement by phenolic additives and the effects of other compounds Pielhop, Thomas; Larrazábal, Gastón O.; Rudolf von Rohr, Philipp Green Chemistry, Vol. 18, Issue 19 https://doi.org/10.1039/C6GC01447J	journal	January 2016
Feeding a sustainable chemical industry: do we have the bioproducts cart before the feedstocks horse? Dale, Bruce E. Faraday Discussions, Vol. 202 https://doi.org/10.1039/C7FD00173H	journal	January 2017
Recent advances in understanding the pseudo-lignin formation in a lignocellulosic biorefinery Shinde, Somnath D.; Meng, Xianzhi; Kumar, Rajeev Green Chemistry, Vol. 20, Issue 10 https://doi.org/10.1039/C8GC00353J	journal	January 2018
Ammonia-salt solvent promotes cellulosic biomass deconstruction under ambient pretreatment conditions to enable rapid soluble sugar production at ultra-low enzyme loadings Chundawat, Shishir P. S.; Sousa, Leonardo da Costa; Roy, Shyamal Green Chemistry, Vol. 22, Issue 1 https://doi.org/10.1039/C9GC03524A	journal	January 2020
Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment Chundawat, Shishir P. S.; Donohoe, Bryon S.; da Costa Sousa, Leonardo Energy & Environmental Science, Vol. 4, Issue 3, 973 https://doi.org/10.1039/c0ee00574f	journal	January 2011
Scaling up and benchmarking of ethanol production from pelletized pilot scale AFEX treated corn stover using Zymomonas mobilis 8b Sarks, Cory; Bals, Bryan D.; Wynn, Jim Biofuels, Vol. 7, Issue 3 https://doi.org/10.1080/17597269.2015.1132368	journal	January 2016
Next-generation cellulosic ethanol technologies and their contribution to a sustainable Africa van Zyl, W. H.; Chimphango, A. F. A.; den Haan, R. Interface Focus, Vol. 1, Issue 2 https://doi.org/10.1098/rsfs.2010.0017	journal	February 2011
Integration in a depot‐based decentralized biorefinery system: Corn stover‐based cellulosic biofuel Kim, Seungdo; Dale, Bruce E.; Jin, Mingjie GCB Bioenergy, Vol. 11, Issue 7 https://doi.org/10.1111/gcbb.12613	journal	January 2019
Activation of crystalline cellulose to cellulose IIII results in efficient hydrolysis by cellobiohydrolase: Hydrolysis of cellulose IIII by cellobiohydrolase Igarashi, Kiyohiko; Wada, Masahisa; Samejima, Masahiro FEBS Journal, Vol. 274, Issue 7 https://doi.org/10.1111/j.1742-4658.2007.05727.x	journal	February 2007
Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose Kristensen, Jan B.; Felby, Claus; Jørgensen, Henning Biotechnology for Biofuels, Vol. 2, Issue 1 https://doi.org/10.1186/1754-6834-2-11	journal	January 2009
Comparing the fermentation performance of Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST) and Zymomonas mobilis AX101 for cellulosic ethanol production Lau, Ming W.; Gunawan, Christa; Balan, Venkatesh Biotechnology for Biofuels, Vol. 3, Issue 1 https://doi.org/10.1186/1754-6834-3-11	journal	January 2010
Effects of alkaline or liquid-ammonia treatment on crystalline cellulose: changes in crystalline structure and effects on enzymatic digestibility Mittal, Ashutosh; Katahira, Rui; Himmel, Michael E. Biotechnology for Biofuels, Vol. 4, Issue 1 https://doi.org/10.1186/1754-6834-4-41	journal	January 2011
A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover Uppugundla, Nirmal; da Costa Sousa, Leonardo; Chundawat, Shishir PS Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-72	journal	January 2014
The correlation between the enzymatic saccharification and the multidimensional structure of cellulose changed by different pretreatments Cui, Ting; Li, Jihong; Yan, Zhipei Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0134-6	journal	September 2014
Sugar loss and enzyme inhibition due to oligosaccharide accumulation during high solids-loading enzymatic hydrolysis Xue, Saisi; Uppugundla, Nirmal; Bowman, Michael J. Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0378-9	journal	November 2015
Techno-economic analysis and climate change impacts of sugarcane biorefineries considering different time horizons Junqueira, Tassia L.; Chagas, Mateus F.; Gouveia, Vera L. R. Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0722-3	journal	March 2017
Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover Gunawan, Christa; Xue, Saisi; Pattathil, Sivakumar Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0757-5	journal	March 2017
Lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier rather than by inducing nonproductive adsorption of enzymes Djajadi, Demi T.; Jensen, Mads M.; Oliveira, Marlene Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1085-0	journal	April 2018
Ethanol production potential from AFEX™ and steam-exploded sugarcane residues for sugarcane biorefineries Mokomele, Thapelo; da Costa Sousa, Leonardo; Balan, Venkatesh Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1130-z	journal	May 2018
Recent developments in biomass pelletization – A review Stelte, Wolfgang; Sanadi, Anand R.; Shang, Lei BioResources, Vol. 7, Issue 3 https://doi.org/10.15376/biores.7.3.Stelte	journal	July 2012
Recent trends on techno-economic assessment (TEA) of sugarcane biorefineries Mandegari, Mohsen Ali; Farzad, Somayeh; Görgens, Johann F. Biofuel Research Journal, Vol. 4, Issue 3 https://doi.org/10.18331/BRJ2017.4.3.7	journal	September 2017
Effect of ammonia fiber expansion on the available energy content of wheat straw fed to lactating cattle and buffalo in India Mor, Preeti; Bals, Bryan; Tyagi, Amrish Kumar Journal of Dairy Science, Vol. 101, Issue 9 https://doi.org/10.3168/jds.2018-14584	journal	September 2018
Shotgun Approach to Increasing Enzymatic Saccharification Yields of Ammonia Fiber Expansion Pretreated Cellulosic Biomass Chundawat, Shishir P. S.; Uppugundla, Nirmal; Gao, Dahai Frontiers in Energy Research, Vol. 5 https://doi.org/10.3389/fenrg.2017.00009	journal	May 2017
Effects of Extractive Ammonia Pretreatment on the Ultrastructure and Glycan Composition of Corn Stover Avci, Utku; Zhou, Xuelian; Pattathil, Sivakumar Frontiers in Energy Research, Vol. 7 https://doi.org/10.3389/fenrg.2019.00085	journal	September 2019
A packed bed Ammonia Fiber Expansion reactor system for pretreatment of agricultural residues at regional depots Campbell, Timothy J.; Teymouri, Farzaneh; Bals, Bryan Biofuels, Vol. 4, Issue 1 https://doi.org/10.4155/bfs.12.71	journal	January 2013

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42 ENGINEERING
AFEX
Engineering
Enzyme dosage
Ethanol
Steam explosion
Sugarcane residues
Uniform feedstock supply

Coupling AFEX and steam-exploded sugarcane residue pellets with a room temperature CIIII-activation step lowered enzyme dosage requirements for sugar conversion

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