The mouse homologue of the tuberin gene (TSC2) maps to a conserved synteny group between mouse chromosome 17 and human 16p13.3
- Lincoln`s Inn Fields, London (United Kingdom); and others
The tuberous sclerosis gene (TSC2) on human chromosome 16p13.3 has recently been identified. Several markers from this region have previously been shown to be members of a conserved synteny group, in the mouse located on chromosome 17. The mouse region includes markers D17Lon1, D17Lon2, D17Lon3, and D17Lon4, which are linked to the {alpha}-globin pseudogene Hba-ps4 on chromosome 17, while the corresponding human markers, NK12, NK92, sazD, and KM17, are linked to the functional {alpha}-globin locus near the tip of chromosome 16p. Since the human TSC2 maps in close proximity to NK12, we wanted to investigate whether a mouse gene, homologous to TSC2, was present on mouse chromosome 17 and thus included in the conserved synteny group. During the characterization of transcripts from the human PKD1 region on human chromosome 16p13.3, we isolated three short clones encoding fragments of TSC2 from a human fetal brain cDNA library enriched for transcripts from the PKD1 region. These TSC2 clones were used as probes to screen a mouse teratocarcinoma (PCC4) cDNA library (Stratagene), at a final stringency of 0.3 x SSC, 0.1% SDS at 65{degrees}C. One of the positive clones isolated, mTS-1, had a 2.8-kb insert. Two hundred bases from each end of the insert were sequenced, showing 88 and 83.5% identity to the human tuberin nucleotide sequence, with the 5{prime} end of the clone starting at position 2351 and the 3{prime} end ending at position 5265. The high degree of homology to the human tuberin sequence suggests that clone mTS-1 is indeed derived from the mouse homologue of TSC2. 11 refs., 1 fig.
- OSTI ID:
- 241112
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 25; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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