Year-around survey and manipulation experiments reveal differential sensitivities of soil prokaryotic and fungal communities to saltwater intrusion in Florida Everglades wetlands

Zhao, Jun; Chakrabarti, Seemanti; Chambers, Randolph; Weisenhorn, Pamela; Travieso, Rafael; Stumpf, Sandro; Standen, Emily; Briceno, Henry; Troxler, Tiffany; Gaiser, Evelyn; Kominoski, John; Dhillon, Braham; Martens-Habbena, Willm

doi:10.1016/j.scitotenv.2022.159865

Year-around survey and manipulation experiments reveal differential sensitivities of soil prokaryotic and fungal communities to saltwater intrusion in Florida Everglades wetlands

Journal Article · Sun Oct 30 00:00:00 EDT 2022 · Science of the Total Environment

DOI:https://doi.org/10.1016/j.scitotenv.2022.159865· OSTI ID:2378599

Zhao, Jun ^[1]; Chakrabarti, Seemanti ^[1]; Chambers, Randolph ^[2]; Weisenhorn, Pamela ^[3]; Travieso, Rafael ^[4]; Stumpf, Sandro ^[4]; Standen, Emily ^[4]; Briceno, Henry ^[4]; Troxler, Tiffany ^[4]; Gaiser, Evelyn ^[4]; Kominoski, John ^[4]; Dhillon, Braham ^[1]; Martens-Habbena, Willm ^[1]

Univ. of Florida, Davie, FL (United States)
College of William and Mary, Williamsburg, VA (United States)
Argonne National Laboratory (ANL), Argonne, IL (United States)
Florida International Univ. (FIU), Miami, FL (United States)

Global sea-level rise is transforming coastal ecosystems, especially freshwater wetlands, in part due to increased episodic or chronic saltwater exposure, leading to shifts in biogeochemistry, plant- and microbial communities, as well as ecological services. Yet, it is still difficult to predict how soil microbial communities respond to the saltwater exposure because of poorly understood microbial sensitivity within complex wetland soil microbial communities, as well as the high spatial and temporal heterogeneity of wetland soils and saltwater exposure. To address this, we first conducted a two-year survey of microbial community structure and bottom water chemistry in submerged surface soils from 14 wetland sites across the Florida Everglades. We identified ecosystem-specific microbial biomarker taxa primarily associated with variation in salinity. Bacterial, archaeal and fungal community composition differed between freshwater, mangrove, and marine seagrass meadow sites, irrespective of soil type or season. Especially, methanogens, putative denitrifying methanotrophs and sulfate reducers shifted in relative abundance and/or composition between wetland types. Methanogens and putative denitrifying methanotrophs declined in relative abundance from freshwater to marine wetlands, whereas sulfate reducers showed the opposite trend. A four-year experimental simulation of salt-water intrusion in a pristine freshwater site and a previously saltwater-impacted site corroborated the highest sensitivity and relative increase of sulfate reducers, as well as taxon-specific sensitivity of methanogens, in response to continuously pulsing of saltwater treatment. Collectively, these results suggest that besides increased salinity, saltwater-mediated increased sulfate availability leads to displacement of methanogens by sulfate reducers even at low or temporal salt exposure. In conclusion, these changes of microbial composition could affect organic matter degradation pathways in coastal freshwater wetlands exposed to sea-level rise, with potential consequences, such as loss of stored soil organic carbon.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 2378599

Journal Information:: Science of the Total Environment, Journal Name: Science of the Total Environment Journal Issue: 1 Vol. 858; ISSN 0048-9697

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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