Tracking alternative versions of the galactose gene network in the genus Saccharomyces and their expansion after domestication

Pontes, Ana; Paraíso, Francisca; Liu, Yu-Ching; Limtong, Savitree; Jindamorakot, Sasitorn; Jespersen, Lene; Gonçalves, Carla; Rosa, Carlos A.; Tsai, Isheng Jason; Rokas, Antonis; Hittinger, Chris Todd; Gonçalves, Paula; Sampaio, José Paulo

doi:10.1016/j.isci.2024.108987

Tracking alternative versions of the galactose gene network in the genus Saccharomyces and their expansion after domestication

Journal Article · Mon Jan 22 23:00:00 EST 2024 · iScience

DOI:https://doi.org/10.1016/j.isci.2024.108987· OSTI ID:2370517

^[1]; Paraíso, Francisca ^[2]; Liu, Yu-Ching ^[3]; Limtong, Savitree ^[4]; Jindamorakot, Sasitorn ^[5]; Jespersen, Lene ^[6]; Gonçalves, Carla ^[2]; Rosa, Carlos A. ^[7]; Tsai, Isheng Jason ^[3]; Rokas, Antonis ^[8]; Hittinger, Chris Todd ^[9]; Gonçalves, Paula ^[2]; Sampaio, José Paulo ^[2]

Nova School of Science and Technology, Caparica (Portugal); Universidade Nova de Lisboa
Nova School of Science and Technology, Caparica (Portugal)
Academia Sinica, Taipei (Taiwan)
Kasetsart University, Bangkok (Thailand); Biodiversity Center Kasetsart University, Bangkok (Thailand)
National Science and Technology, Development Agency (NSTDA), Pathum Thani (Thailand)
University of Copenhagen (Denmark)
Universidade Federal de Minas Gerais (Brazil)
Vanderbilt University, Nashville, TN (United States)
University of Wisconsin-Madison, WI (United States)

When Saccharomyces cerevisiae grows on mixtures of glucose and galactose, galactose utilization is repressed by glucose, and induction of the GAL gene network only occurs when glucose is exhausted. Contrary to reference GAL alleles, alternative alleles support faster growth on galactose, thus enabling distinct galactose utilization strategies maintained by balancing selection. Here, we report on new wild populations of Saccharomyces cerevisiae harboring alternative GAL versions and, for the first time, of Saccharomyces paradoxus alternative alleles. We also show that the non-functional GAL version found earlier in Saccharomyces kudriavzevii is phylogenetically related to the alternative versions, which constitutes a case of trans-specific maintenance of highly divergent alleles. Strains harboring the different GAL network variants show different levels of alleviation of glucose repression and growth proficiency on galactose. We propose that domestication involved specialization toward thriving in milk from a generalist ancestor partially adapted to galactose consumption in the plant niche.

View Accepted Manuscript (DOE)

Research Organization:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); Fundação para a Ciência e a Tecnologia (Portugal)

Grant/Contract Number:: SC0018409

OSTI ID:: 2370517

Journal Information:: iScience, Journal Name: iScience Journal Issue: 2 Vol. 27; ISSN 2589-0042

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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