Exogenous erythropoietin increases hematological status, fat oxidation, and aerobic performance in males following prolonged strenuous training

Drummer, Devin J.; McNiff, Julie L.; Howard, Emily E.; Gwin, Jess A.; Carrigan, Christopher T.; Murphy, Nancy E.; Wilson, Marques A.; Michalak, Julia; Ryan, Benjamin J.; McClung, James P.; Pasiakos, Stefan M.; Margolis, Lee M.

doi:10.14814/phy2.16038

Exogenous erythropoietin increases hematological status, fat oxidation, and aerobic performance in males following prolonged strenuous training

Journal Article · Thu May 16 00:00:00 EDT 2024 · Physiological Reports

DOI:https://doi.org/10.14814/phy2.16038· OSTI ID:2352333

Drummer, Devin J. ^[1]; McNiff, Julie L. ^[2]; Howard, Emily E. ^[3]; Gwin, Jess A. ^[3]; Carrigan, Christopher T. ^[3]; Murphy, Nancy E. ^[3]; Wilson, Marques A. ^[3]; Michalak, Julia ^[1]; Ryan, Benjamin J. ^[4]; McClung, James P. ^[3]; Pasiakos, Stefan M. ^[5]; ^[3]

Military Nutrition Division U.S. Army Research Institute of Environmental Medicine Natick Massachusetts USA, Oak Ridge Institute for Science and Education Belcamp Maryland USA
Military Nutrition Division U.S. Army Research Institute of Environmental Medicine Natick Massachusetts USA, Combat Feeding Division U.S. Army Combat Capabilities Development Command (DEVCOM) Natick Massachusetts USA
Military Nutrition Division U.S. Army Research Institute of Environmental Medicine Natick Massachusetts USA
Thermal and Mountain Medicine Division U.S. Army Research Institute of Environmental Medicine Natick Massachusetts USA
Office of Dietary Supplements, National Institutes of Health U.S. Department of Health and Human Services Bethesda Maryland USA

Abstract

This study investigated the effects of EPO on hemoglobin (Hgb) and hematocrit (Hct), time trial (TT) performance, substrate oxidation, and skeletal muscle phenotype throughout 28 days of strenuous exercise. Eight males completed this longitudinal controlled exercise and feeding study using EPO (50 IU/kg body mass) 3×/week for 28 days. Hgb, Hct, and TT performance were assessed PRE and on Days 7, 14, 21, and 27 of EPO. Rested/fasted muscle obtained PRE and POST EPO were analyzed for gene expression, protein signaling, fiber type, and capillarization. Substrate oxidation and glucose turnover were assessed during 90‐min of treadmill load carriage (LC; 30% body mass; 55 ± 5% V̇O ₂ peak) exercise using indirect calorimetry, and 6‐6‐[ ² H ₂ ]‐glucose PRE and POST. Hgb and Hct increased, and TT performance improved on Days 21 and 27 compared to PRE ( p < 0.05). Energy expenditure, fat oxidation, and metabolic clearance rate during LC increased ( p < 0.05) from PRE to POST. Myofiber type, protein markers of mitochondrial biogenesis, and capillarization were unchanged PRE to POST. Transcriptional regulation of mitochondrial activity and fat metabolism increased from PRE to POST ( p < 0.05). These data indicate EPO administration during 28 days of strenuous exercise can enhance aerobic performance through improved oxygen carrying capacity, whole‐body and skeletal muscle fat metabolism.

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Sponsoring Organization:: USDOE

OSTI ID:: 2352333

Alternate ID(s):: OSTI ID: 2352334

Journal Information:: Physiological Reports, Journal Name: Physiological Reports Journal Issue: 10 Vol. 12; ISSN 2051-817X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United States

Language:: English

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