Structure of the antenna complex expressed during far-red light photoacclimation in Synechococcus sp. PCC 7335

Gisriel, Christopher J.; Shen, Gaozhong; Brudvig, Gary W.; Bryant, Donald A.

doi:10.1016/j.jbc.2023.105590

Structure of the antenna complex expressed during far-red light photoacclimation in Synechococcus sp. PCC 7335

Journal Article · Fri Dec 22 00:00:00 EST 2023 · Journal of Biological Chemistry

DOI:https://doi.org/10.1016/j.jbc.2023.105590· OSTI ID:2339817

^[1]; Shen, Gaozhong ^[2]; Brudvig, Gary W. ^[3]; Bryant, Donald A. ^[2]

Yale University, New Haven, CT (United States); Yale University; Yale University
Pennsylvania State University, University Park, PA (United States)
Yale University, New Haven, CT (United States)

Far-red light photoacclimation, or FaRLiP, is a facultative response exhibited by some cyanobacteria that allows them to absorb and utilize lower energy light (700–800 nm) than the wavelengths typically used for oxygenic photosynthesis (400–700 nm). During this process, three essential components of the photosynthetic apparatus are altered: photosystem I, photosystem II, and the phycobilisome. In all three cases, at least some of the chromophores found in these pigment–protein complexes are replaced by chromophores that have red-shifted absorbance relative to the analogous complexes produced in visible light. Recent structural and spectroscopic studies have elucidated important features of the two photosystems when altered to absorb and utilize far-red light, but much less is understood about the modified phycobiliproteins made during FaRLiP. We used single-particle, cryo-EM to determine the molecular structure of a phycobiliprotein core complex comprising allophycocyanin variants that absorb far-red light during FaRLiP in the marine cyanobacterium Synechococcus sp. PCC 7335. The structure reveals the arrangement of the numerous red-shifted allophycocyanin variants and the probable locations of the chromophores that serve as the terminal emitters in this complex. It also suggests how energy is transferred to the photosystem II complexes produced during FaRLiP. The structure additionally allows comparisons with other previously studied allophycocyanins to gain insights into how phycocyanobilin chromophores can be tuned to absorb far-red light. These studies provide new insights into how far-red light is harvested and utilized during FaRLiP, a widespread cyanobacterial photoacclimation mechanism.

View Accepted Manuscript (DOE)

Research Organization:: Yale University, New Haven, CT (United States)

Sponsoring Organization:: National Institutes of Health (NIH); National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FG02-05ER15646

OSTI ID:: 2339817

Journal Information:: Journal of Biological Chemistry, Journal Name: Journal of Biological Chemistry Journal Issue: 2 Vol. 300; ISSN 0021-9258

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
FaRLiP
allophycocyanin
cryo-EM
cyanobacteria
energy transfer
far-red light photoacclimation
light harvesting
photosynthesis
phycobilisome
phycocyanobilin

Structure of the antenna complex expressed during far-red light photoacclimation in Synechococcus sp. PCC 7335

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