Helical allophycocyanin nanotubes absorb far-red light in a thermophilic cyanobacterium

Gisriel, Christopher J.; Elias, Eduard; Shen, Gaozhong; Soulier, Nathan T.; Flesher, David A.; Gunner, M. R.; Brudvig, Gary W.; Croce, Roberta; Bryant, Donald A.

doi:10.1126/sciadv.adg0251

Helical allophycocyanin nanotubes absorb far-red light in a thermophilic cyanobacterium

Journal Article · Fri Mar 24 00:00:00 EDT 2023 · Science Advances

DOI:https://doi.org/10.1126/sciadv.adg0251· OSTI ID:2339809

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Yale Univ., New Haven, CT (United States); Yale University; Yale University
Vrije Univ., Amsterdam (Netherlands)
Pennsylvania State Univ., University Park, PA (United States)
Yale Univ., New Haven, CT (United States)
City College of New York, NY (United States)

To compete in certain low-light environments, some cyanobacteria express a paralog of the light-harvesting phycobiliprotein, allophycocyanin (AP), that strongly absorbs far-red light (FRL). Using cryo–electron microscopy and time-resolved absorption spectroscopy, we reveal the structure-function relationship of this FRL-absorbing AP complex (FRL-AP) that is expressed during acclimation to low light and that likely associates with chlorophyll a–containing photosystem I. FRL-AP assembles as helical nanotubes rather than typical toroids due to alterations of the domain geometry within each subunit. Spectroscopic characterization suggests that FRL-AP nanotubes are somewhat inefficient antenna; however, the enhanced ability to harvest FRL when visible light is severely attenuated represents a beneficial trade-off. The results expand the known diversity of light-harvesting proteins in nature and exemplify how biological plasticity is achieved by balancing resource accessibility with efficiency.

View Accepted Manuscript (DOE)

Research Organization:: Yale Univ., New Haven, CT (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: FG02-05ER15646; SC0001423

OSTI ID:: 2339809

Journal Information:: Science Advances, Journal Name: Science Advances Journal Issue: 12 Vol. 9; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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