Structure of a dimeric photosystem II complex from a&nbsp;cyanobacterium acclimated to far-red light

Gisriel, Christopher J.; Shen, Gaozhong; Flesher, David A.; Kurashov, Vasily; Golbeck, John H.; Brudvig, Gary W.; Amin, Muhamed; Bryant, Donald A.

doi:10.1016/j.jbc.2022.102815

Structure of a dimeric photosystem II complex from a cyanobacterium acclimated to far-red light

Journal Article · Tue Dec 20 00:00:00 EST 2022 · Journal of Biological Chemistry

DOI:https://doi.org/10.1016/j.jbc.2022.102815· OSTI ID:2339806

^[1]; Shen, Gaozhong ^[2]; Flesher, David A. ^[3]; Kurashov, Vasily ^[2]; Golbeck, John H. ^[2]; Brudvig, Gary W. ^[3]; ^[4]; Bryant, Donald A. ^[2]

Yale Univ., New Haven, CT (United States); Yale University; Yale University
Pennsylvania State Univ., University Park, PA (United States)
Yale Univ., New Haven, CT (United States)
University of Groningen (Netherlands); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)

Photosystem II (PSII) is the water-splitting enzyme central to oxygenic photosynthesis. To drive water oxidation, light is harvested by accessory pigments, mostly chlorophyll (Chl) a molecules, which absorb visible light (400–700 nm). Some cyanobacteria facultatively acclimate to shaded environments by altering their photosynthetic machinery to additionally absorb far-red light (FRL, 700–800 nm), a process termed far-red light photoacclimation or FaRLiP. During far-red light photoacclimation, FRL-PSII is assembled with FRL-specific isoforms of the subunits PsbA, PsbB, PsbC, PsbD, and PsbH, and some Chl-binding sites contain Chls d or f instead of the usual Chl a. The structure of an apo-FRL-PSII monomer lacking the FRL-specific PsbH subunit has previously been determined, but visualization of the dimeric complex has remained elusive. Here, we report the cryo-EM structure of a dimeric FRL–PSII complex. The site assignments for Chls d and f are consistent with those assigned in the previous apo-FRL-PSII monomeric structure. All sites that bind Chl d or Chl f at high occupancy exhibit a FRL-specific interaction of the formyl moiety of the Chl d or Chl f with the protein environment, which in some cases involves a phenylalanine sidechain. The structure retains the FRL-specific PsbH2 subunit, which appears to alter the energetic landscape of FRL-PSII, redirecting energy transfer from the phycobiliprotein complex to a Chl f molecule bound by PsbB2 that acts as a bridge for energy transfer to the electron transfer chain. Collectively, these observations extend our previous understanding of the structure-function relationship that allows PSII to function using lower energy FRL.

View Accepted Manuscript (DOE)

Research Organization:: Yale Univ., New Haven, CT (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: FG02-05ER15646

OSTI ID:: 2339806

Journal Information:: Journal of Biological Chemistry, Journal Name: Journal of Biological Chemistry Journal Issue: 1 Vol. 299; ISSN 0021-9258

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
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cyanobacteria
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Structure of a dimeric photosystem II complex from a cyanobacterium acclimated to far-red light

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