Two-Dimensional Electronic Spectroscopy of the Far-Red-Light Photosystem II Reaction Center

Silori, Yogita; Willow, Rhiannon; Nguyen, Hoang H.; Shen, Gaozhong; Song, Yin; Gisriel, Christopher J.; Brudvig, Gary W.; Bryant, Donald A.; Ogilvie, Jennifer P.

doi:10.1021/acs.jpclett.3c02604

Two-Dimensional Electronic Spectroscopy of the Far-Red-Light Photosystem II Reaction Center

Journal Article · Thu Nov 09 00:00:00 EST 2023 · Journal of Physical Chemistry Letters

DOI:https://doi.org/10.1021/acs.jpclett.3c02604· OSTI ID:2337792

Silori, Yogita ^[1]; ^[2]; Nguyen, Hoang H. ^[2]; Shen, Gaozhong ^[3]; ^[4]; ^[5]; ^[5]; Bryant, Donald A. ^[3]; ^[2]

Univ. of Michigan, Ann Arbor, MI (United States); University of Michigan
Univ. of Michigan, Ann Arbor, MI (United States)
Pennsylvania State Univ., University Park, PA (United States)
Beijing Institute of Technology (China)
Yale Univ., New Haven, CT (United States)

Understanding the role of specific pigments in primary energy conversion in the photosystem II (PSII) reaction center has been impeded by the spectral overlap of its constituent pigments. When grown in far-red light, some cyanobacteria incorporate chlorophyll-f and chlorophyll-d into PSII, relieving the spectral congestion. Here we employ two-dimensional electronic spectroscopy to study PSII at 77 K from Synechococcus sp. PCC 7335 cells that were grown in far-red light (FRL-PSII). We observe the formation of a radical pair within ~3 ps that we assign to Chl_D1^•–P_D1^•+. While Pheo_D1 is thought to act as the primary electron acceptor in PSII from cells grown in visible light, we see no evidence of its involvement, which we attribute to its reduction by dithionite treatment in our samples. Our work demonstrates that primary charge separation occurs between Chl_D1 and P_D1 in FRL-PSII, suggesting that P_D1/P_D2 may play an underappreciated role in PSII’s charge separation mechanism.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Michigan, Ann Arbor, MI (United States)

Sponsoring Organization:: National Institutes of General Medical Sciences (NIGMS); National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: FG02-05ER15646; SC0016384

OSTI ID:: 2337792

Journal Information:: Journal of Physical Chemistry Letters, Journal Name: Journal of Physical Chemistry Letters Journal Issue: 45 Vol. 14; ISSN 1948-7185

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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