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OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Constructing the Nitrogen Flux Maps (NFMs) of Plants

Technical Report ·
DOI:https://doi.org/10.2172/2326084· OSTI ID:2326084
 [1];  [2];  [2];  [3];  [4]
  1. Univ. of Wisconsin, Madison, WI (United States)
  2. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
  3. Hokkaido Univ., Sapporo (Japan)
  4. Univ. of Potsdam (Germany)
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The main objectives of this project are to construct plant N flux maps (NFMs) from plant genomes and to determine functionality of AT enzymes and plant N metabolic network. To address this grand challenge, this project made use of rapidly growing numbers of plant genomes, high-throughput functional characterization platforms, and computational modeling to deduce both biochemical and systems level functionality of ATs and NFMs. The obtained NFMs will provide a novel framework to advance basic understanding of plant N metabolism and facilitate rational engineering of plants with high productivity even under limited N input.

Research Organization:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
DOE Contract Number:
SC0020390
OSTI ID:
2326084
Report Number(s):
DE-SC0020390-MADISON-FINAL
Country of Publication:
United States
Language:
English

References (8)

VAS1-mediated nitrogen reshuffling in aromatic amino acid homeostasis March 2024
Evolutionary origin and functional diversification of aminotransferases August 2022
Multi-substrate specificity shaped the complex evolution of the aminotransferase family across the tree of life March 2024
Systematic comparison of local approaches for isotopically nonstationary metabolic flux analysis June 2023
Mass spectrometry imaging–based assays for aminotransferase activity reveal a broad substrate spectrum for a previously uncharacterized enzyme March 2023
Microbiome engineering for sustainable agriculture: using synthetic biology to enhance nitrogen metabolism in plant-associated microbes August 2022
An automated workflow that generates atom mappings for large‐scale metabolic models and its application to Arabidopsis thaliana July 2022
Biochemical characterization of plant aromatic aminotransferases January 2023

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59 BASIC BIOLOGICAL SCIENCES
nitrogen metabolic network
aminotransferase
transaminase
enzyme evolution
metabolic modeling
substrate specificity