miR-126 regulates angiogenesis in myocardial ischemia by targeting HIF-1α

Journal Article · Wed Dec 15 04:00:00 EST 2021 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2021.112925· OSTI ID:23195275

Gao, Shuibo; Gao, Haixia ^[1]; Dai, Liping ^[2]; Han, Yongjun; Lei, Zhen ^[1]; others, and

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1. Second School of Clinical Medicine, Henan University of Chinese Medicine, Zhengzhou, 450002 (China)
2. School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046 (China)

Promoting angiogenesis by targeting various angiogenic regulators has emerged as a new treatment strategy for myocardial ischemia (MI). MicroRNA-126 (miR-126) has been identified as the main regulator of compensatory angiogenesis; however, its role in MI is unclear. A rat MI model and an EA. hy926 endothelial cell hypoxia model were constructed and it was found that miR-126 was highly expressed in both models. The knockdown of HIF-1α expression in EA. hy926 cells in turn downregulated VEGF and CD34 expression and consequently inhibited angiogenesis. MiR-126 inhibitor inhibited EA. hy926 cell migration and tube formation as well as downregulated VEGF and CD34 expression, and these were reversed by transfection of miR-126 mimics. Rescue tests using miR-126 and HIF-1α demonstrated that miR-126-mediated regulation of angiogenesis was dependent on HIF-1α. In summary, miR-126 regulates the occurrence and progression of angiogenesis during MI via HIF-1α and may be a potential new therapeutic target.

• OSTI ID: 23195275
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 2 Vol. 409; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANGIOGENESIS
ANOXIA
ISCHEMIA
RATS