Analysis of T cell receptor repertoire in monozygotic twins concordant and discordant for chronic hepatitis B infection
- Institute of Immunology, PLA, Third Military Medical University, Chongqing 400038 (China)
- Institute of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038 (China)
- Department of Health, Third Military Medical University, Chongqing 400038 (China)
Highlights: • TCR Repertoire features from twin pairs show heritable bias. • TCR repertoires show antigen biases in chronic viral infection patients. • TCR repertoires show a high level of concordance among HBeAg-positive HBV twins. • Shared clonotypes in identical cured twins show therapeutic potential. Due to their identical inheritance and shared surroundings, identical twins have been the recommended group for studying the susceptibility and prognosis of diseases. Here, CD8{sup +} T cell receptor beta (TCRβ) chains were analyzed by high-throughput sequencing in three pairs of healthy identical twins and chronic hepatitis B patients. The data showed a high level of similarity in the TCR repertoire of each pair in terms of average TCR Vβ segment expression and frequency of the complementary determining region 3 (CDR3) pattern and skewed or oligoclonal clonotypes. Notably, the level of similarity in TCR Vβ expression between the twins appeared to be independent of the consistency or inconsistency of chronic HBV infection, although the detailed CDR3 pattern and frequency were related to disease prognosis. There were more immunodominant clonotypes in patients with HBV antigen seroconversion, which showed an increased abundance. These immunodominant clonotypes may be used as favorable prognostic biomarkers and potential targets for immunotherapy. Thus, delineating the CD8{sup +} T cell repertoire of identical twins with concordant chronic viral infections provides a promising means to screen protective TCR genes for immunotherapy.
- OSTI ID:
- 23137339
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 497; ISSN BBRCA9; ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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