Knockdown of long non-coding RNA MAP3K20 antisense RNA 1 inhibits gastric cancer growth through epigenetically regulating miR-375

Journal Article · Thu Mar 15 00:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.02.072· OSTI ID:23137303

Quan, Yongsheng ^[1]; Zhang, Yan ^[2]; Lin, Wei ^[3]; Shen, Zhaohua; Wu, Shuai; Zhu, Changxin; Wang, Xiaoyan ^[1]

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1. Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan (China)
2. Department of Ultrasound, The Third Xiangya Hospital of Central South University, Changsha, Hunan (China)
3. Department of Pathology, Xiangya Hospital of Central South University, Changsha, Hunan (China)

Highlights: • lncRNA MLK7-AS1 is an independent prognostic factor for gastric cancer patients. • Knockdown of MLK7-AS1 inhibits gastric cancer cell growth. • MLK7-AS1 negatively and epigenetically controls miR-375 expression. Emerging evidence has demonstrated that long noncoding RNAs (lncRNAs) play a critical role in tumorigenesis of gastric cancer. LncRNA MAP3K20 antisense RNA 1 (MLK7-AS1) has been identified as one of gastric cancer-specific lncRNAs. However, its precise role in gastric cancer remains unknown. In this study, we found that lncRNA MLK7-AS1 was significantly increased in gastric cancer tissues compared with in adjacent tissues. Gastric cancer patients with high MLK7-AS1 expression had a shorter survival and poorer prognosis. By loss-function assay, we demonstrated that knockdown of MLK7-AS1 inhibited cell proliferation and induced apoptosis in HGC27and MKN-45 cells. Furthermore, we identified miR-375 as a target of MLK7-AS1. MLK7-AS1 interacted with Dnmt1 and recruited it to miR-375 promotor, hyper-methylating miR-375 promotor and repressing miR-375 expression. Taken together, our findings demonstrate that knockdown of MLK7-AS1 by siRNA inhibits gastric cancer growth by epigenetically regulating miR-375. Thus, MLK7-AS1 may be a useful prognostic marker and therapeutic target for gastric cancer patients.

• OSTI ID: 23137303
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 497, Issue 2; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
CELL PROLIFERATION
NEOPLASMS
RNA