The suppressive effect of puerarin on atopic dermatitis-like skin lesions through regulation of inflammatory mediators in vitro and in vivo
- Department of Immunology and Institute for Medical Sciences, Chonbuk National University Medical School, Jeonju, Jeollabuk-do 54907 (Korea, Republic of)
- Department of Oriental Medicine Resources, Chonbuk National University, Iksan, Jeollabuk-do 54596 (Korea, Republic of)
- Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk, 54538 (Korea, Republic of)
Highlights: • Puerarin regulates inflammatory cytokine, such as IL-6, IL-5, and TNF-α in human keratinocyte cell line. • Puerarin attenuates inflammation related protein expression level in human keratinocyte cell line. • Puerarin improves DNCB-induced atopic skin condition by regulating of clinical signs and skin tissue injury. • Oral administration of puerarin recovers inflammatory cytokine secretion in DNCB-induced atopic dermatitis. Atopic dermatitis (AD) is one of the common inflammatory immune disorders. Puerarin is the main isoflavonoid obtained from the root of Pueraria lobata and has been known have ameliorative effects on diverse inflammatory diseases. However, the effects of puerarin on AD have not been uncovered. 2,4-dinitrochlorobenzene (DNCB) was used to induce atopic dermatitis(AD)-like skin lesions on BALB/c mice for 17 days. Further, the BALB/c mice were orally administered puerarin. Puerarin ameliorated DNCB-induced AD-like symptoms in the mice by regulating skin thickness, degranulation of mast cells, and serum immunoglobulin E (IgE). Human keratinocytes (HaCaT cells) were also used to clarify the effects of puerarin on the secretion of pro-inflammatory cytokines. Puerarin inhibited the secretion of inflammatory cytokines and chemokines. The aim of this study was to investigate the protective and alleviative effect of puerarin on AD in vitro and in vivo. The results in this study indicated that puerarin ameliorates AD-like skin lesion and skin inflammation via regulation of various atopic and inflammatory mediators. Therefore, puerarin might be useful in treating AD and other skin diseases.
- OSTI ID:
- 23137223
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 498, Issue 4; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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