Adipose-derived stem cells ameliorate colitis by suppression of inflammasome formation and regulation of M1-macrophage population through prostaglandin E2
- Department of Internal Medicine, Yonsei University, Wonju, South (Korea, Republic of)
- Department of Plastic and Reconstructive Surgery, Yonsei University, Wonju, South (Korea, Republic of)
- Cell Therapy and Tissue Engineering Center, Wonju College of Medicine, Yonsei University, Wonju, South (Korea, Republic of)
- Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, South (Korea, Republic of)
Highlights: • PGE2 was remarkably increased in conditioned media from THP-1 co-cultured with ASCs. • ASCs induced M1 to M2 macrophage transition through PGE2. • ASCs suppressed secretion of inflammatory IL-1β and IL-18 through PGE2. • ASCs ameliorated colitis by decreasing the total number of macrophages. • ASCs relieved inflammation by decreasing the M1 macrophage population. Inflammatory bowel disease (IBD) is an idiopathic disease caused by a dysregulated immune response to intestinal microbes in an individual with a genetic predisposition. Therefore, alleviation of inflammation is very important to treat IBD. Mesenchymal stem cells (MSCs) have been highlighted as new candidates for treating autoimmune disease based on their immunomodulatory properties. In this study, we investigated the anti-inflammatory mechanism and therapeutic effects of adipose tissue-derived MSCs (ASCs) using THP-1 macrophages and dextran sodium sulfate (DSS)-induced mice with chronic colitis. LPS-treated THP-1 cells expressed mRNA of CD11b, an M1 macrophage marker, at day 2. However, THP-1 co-cultured with ASCs expressed mRNA of CD206, CD68, CCL18, legumain, and IL-10, markers of M2 macrophages. In THP-1 cells co-cultured with ASCs, precursor (pro)-IL-1β, Cox-2, and NLRP3 increased dramatically compared to LPS-treated THP-1 cells. Secretion of IL-1β and IL-18 was significantly inhibited by ASCs, but PGE2 production was highly increased in co-culture conditions of THP-1 and ASCs. IL-18 secretion was inhibited by PGE2 treatment, and PGE2 inhibited inflammasome complex (ASC/Cas-1/NLRP3) formation in THP-1 cells. In the DSS-induced chronic colitis model, ASCs ameliorated colitis by decreasing the total number of macrophages and the M1 macrophage population. Our results suggest that ASCs can suppress the inflammatory response by controlling the macrophage population, and ASCs may be therapeutically useful for the treatment of IBD.
- OSTI ID:
- 23137219
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 498, Issue 4; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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