RBM14 is indispensable for pluripotency maintenance and mesoderm development of mouse embryonic stem cells

Journal Article · Fri Jun 15 00:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.04.231· OSTI ID:23137071

Chen, Guilai ^[1]; Zhang, Da ^[1]; Zhang, Linlin; Feng, Guihai ^[1]; Zhang, Boya ^[1]; Wu, Yihui; Li, Wei; Zhang, Ying; Hu, Baoyang ^[1]

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1. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101 (China)

Highlights: • RBM14 knockout mouse embryonic stem cells were successfully generated. • RBM14 depletion altered gene expression profiles in mouse embryonic stem cells. • RBM14 depletion impeded mesoderm development in vitro. • RBM14 depletion impeded mesoderm development in vivo. The pluripotency of embryonic stem cells (ESCs) is maintained by core pluripotency transcription factors, cofactors and several signaling pathways. RBM14 is a component of the para-speckle complex, which has been implicated in multiple important biological processes. The role of RBM14 in ESCs and lineage differentiation remains to be elucidated. In the present study, we provided evidence that RBM14 plays important roles in maintaining pluripotency and in the early differentiation of ESCs. RBM14 was demonstrated to be expressed in mouse embryonic stem cells (mESCs) and localized in the nucleus. RBM14 expression was depleted in mESCs using clustered regularly interspaced short palindromic repeats (CRISPR) technology. Our results also showed that RBM14 depletion altered the gene expression profiles of mESCs. In particular, pluripotency-associated genes and genes involved in the Wnt and TGF-β signaling pathways were downregulated in RBM14 knockout mESCs. Furthermore, RBM14 was found to be essential for mesoderm development in vitro and in vivo. The specific effects of RBM14 depletion were verified by conducting a rescue experiment. Our findings demonstrated that RBM14 not only plays an important role in maintaining the pluripotency of mESCs but is also indispensable for mesoderm development.

• OSTI ID: 23137071
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 501, Issue 1; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
IN VITRO
IN VIVO
MICE
STEM CELLS
TRANSCRIPTION FACTORS