Overexpressing circular RNA hsa{sub c}irc{sub 0}002052 impairs osteosarcoma progression via inhibiting Wnt/β-catenin pathway by regulating miR-1205/APC2 axis

Journal Article · Wed Aug 15 04:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.05.184· OSTI ID:23136959

Wu, Zhen ^[1]; Shi, Wangping ^[2]; Jiang, Chendi ^[3]

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1. The Orthopaedic Department of Tongde Hospital of Zhejiang Province, Hangzhou, 310012 (China)
2. Department of Pharmacy, Zhejiang Medical & Health Group Hangzhou Hospital, Hangzhou, 310022 (China)
3. Department of Orthopaedic Surgery, The Dingli Clinical Institute of Wenzhou Medical University, Wenzhou, 325000 (China)

Circular RNAs (circRNAs) are a novel class of noncoding RNAs, whose importance in cancer has been gradually acknowledged. However, the functions of circRNAs in tumorigenesis have not been fully understood. In the present study, we identified a novel circRNA hsa{sub c}irc{sub 0}002052 significantly downregulated in osteosarcoma (OS) tissues and cell lines. Moreover, we found that hsa{sub c}irc{sub 0}002052 could act as a biomarker to indicate the prognosis of OS patients. Functionally, we showed that hsa{sub c}irc{sub 0}002052 overexpression significantly suppressed OS cell proliferation, migration and invasion while promoting apoptosis in vitro. Similarly, in vivo assay indicated that ectopic expression of hsa{sub c}irc{sub 0}002052 impaired OS cell growth. In terms of mechanism, we found that hsa{sub c}irc{sub 0}002052 inhibited miR-1205 while miR1205 targeted APC2, a negative regulator of Wnt/β-catenin signaling pathway. By releasing the inhibition of miR-1205 on APC2 expression, hsa{sub c}irc{sub 0}002052 suppressed the activation of Wnt/β-catenin signaling pathway, leading to attenuated OS progression. Taken together, our study for the first time revealed a suppressive circRNA hsa{sub c}irc{sub 0}002052 involved in OS progression. Our study suggested hsa{sub c}irc{sub 0}002052/miR-1205/APC2/Wnt/β-catenin axis might be a potential target for OS therapy.

• OSTI ID: 23136959
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 502; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
BIOLOGICAL MARKERS
CELL PROLIFERATION
OSTEOSARCOMAS
RNA