Inhibition of TBK1 reduces choroidal neovascularization in vitro and in vivo
- School of Medicine, Nantong University, Nantong, 226001, Jiangsu (China)
- Nantong University, Nantong, Jiangsu (China)
- Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226001, Jiangsu (China)
- Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, 226001 (China)
- Department of Ophthalmology, The First People's Hospital of Nantong, 226001, Jiangsu Province (China)
Highlights: • The expression of TBK1 and VEGF is up-regulated in CNV model. • Knockdown of TBK1 alleviates CNV formation via inhibiting VEGF expression. • TBK1 plays a critical role in the development of CNV. choroidal neovascularization (CNV), a characteristic of wet age-related macular degeneration (AMD), causes severe vision loss among elderly patients. TANK-binding kinase 1 (TBK1) is a ubiquitously expressed serine–threonine kinase and is found to induce endothelial cells proliferation, represent a novel mediator of tumor angiogenesis and exert pro-inflammatory effect. However, the role of TBK1 in choroidal neovascularization has not been investigated so far. In this study, we found that the expression of TBK1 and VEGF was up-regulated in RF/6 A cells chemical hypoxia model and laser-induced mouse CNV model. Silencing of TBK1 suppressed the proliferation and tube formation activity of RF/6 A cells. Intravitreal injection of anti-TBK1 monoclonal antibody ameliorates CNV formation. Taken together, these findings exhibit a proangiogenic role for TBK1 via upregulating the expression of VEGF, and may suggest that TBK1 inhibition offers a unique and alternative method for prevention and treatment of AMD.
- OSTI ID:
- 23136939
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 503, Issue 1; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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