Chd1p recognizes H3K36Ac to maintain nucleosome positioning near the transcription start site

Journal Article · Sat Sep 15 04:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.07.025· OSTI ID:23134351

Kim, Hyunhee; Jo, Hyelim; Seo, Hogyu David ^[1]; Park, Hee-Sung ^[2]; Lee, Daeyoup ^[1]

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1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, South (Korea, Republic of)
2. Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, South (Korea, Republic of)

In Saccharomyces cerevisiae, the ATP-dependent chromatin remodeler, Chd1p, globally affects nucleosome positioning at coding regions, where nucleosomes are specifically and directionally aligned with respect to the transcription start site (TSS). Various auxiliary domains of remodelers play critical roles by performing specialized functions that are unique to the type of remodeler. Here, we report that yeast Chd1p directly binds to acetylated histone H3K36 (H3K36Ac) via its chromodomain, and that H3K36Ac stimulates the nucleosome sliding activity of Chd1p in vitro. Furthermore, we use genome-wide analysis to demonstrate that H3K36Ac promotes the remodeling activity of Chd1p to maintain chromatin stability at the 5′ ends of genes in vivo. Our work linking Chd1p with H3K36Ac provides novel insights into how the nucleosome remodeling activity of Chd1p is controlled near the TSS.

• OSTI ID: 23134351
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 503; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ATP
HISTONES
NUCLEOSOMES
SACCHAROMYCES CEREVISIAE