Minocycline prevents peroxidative permeabilization of cardiolipin-containing bilayer lipid membranes mediated by cytochrome c
- Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991 (Russian Federation)
Highlights: • Minocycline blocked cytochrome c-mediated peroxidative permeabilization of liposomes. • Minocycline prevented cytochrome c binding to cardiolipin-containing liposomes. • Minocycline suppressed cardiolipin-stimulated peroxidase activity of cytochrome c. • This could be relevant to minocycline inhibition of cyt c release from mitochondria. Peroxidase activity of cytochrome c stimulated by interaction of the protein with cardiolipin is considered to be involved in the induction of mitochondrial apoptosis associated with cytochrome c release from mitochondria. In model systems, this activity has been previously shown to cause permeabilization of cardiolipin-containing membranes. Here, we found that the antibiotic minocycline, known to have neuroprotective properties, inhibited both the binding of cyt c to cardiolipin-containing membranes and the cyt c/H{sub 2}O{sub 2}-induced liposome permeabilization. The results could be relevant to inhibition of cyt c release from mitochondria exerted by minocycline.
- OSTI ID:
- 23134288
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1-4 Vol. 507; ISSN 0006-291X; ISSN BBRCA9
- Country of Publication:
- United States
- Language:
- English