Global identification of circular RNAs in chronic myeloid leukemia reveals hsa{sub c}irc{sub 0}080145 regulates cell proliferation by sponging miR-29b

Journal Article · Mon Oct 15 04:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.08.154· OSTI ID:23134185

Liu, Jianhua ^[1]; Kong, Fanjiu ^[2]; Lou, Shuhang ^[1]; Yang, Dongchang ^[3]; Gu, Linping ^[4]

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1. Department of Blood Transfusion, Linyi Central Hospital, Linyi (China)
2. Department of Blood Transfusion, The First Hospital of Zibo, Zibo (China)
3. Department of Blood Transfusion, Binzhou Central Hospital, Binzhou (China)
4. Department of Hematology, Jinan Central Hospital, Jinan (China)

Highlights: • A large number of circRNAs aberrantly expressed in chronic myeloid leukemia. • Top 20 aberrantly expressed interacted with leukemia-associated miRNAs. • hsa{sub c}irc{sub 0}080145 knockdown significantly suppressed CML cell proliferation. • hsa{sub c}irc{sub 0}080145 acts as an miR-29b sponge in CML. Circular RNAs (circRNAs) are a class of non-coding RNAs that participate in various biological processes and disease pathogenesis. However, the role of circRNAs in chronic myeloid leukemia (CML) remains largely unknown. In this study, high-throughput sequencing was performed to explore the expression profile of circRNAs in CML patients for the first time, and a large number of differentially expressed circRNAs were identified. In addition, we constructed potential circRNA-miRNA interaction networks in CML, including a detailed hsa{sub c}irc{sub 0}080145-mediated competing endogenous RNA (ceRNA) regulatory network. Furthermore, functional experiments demonstrated that hsa{sub c}irc{sub 0}080145 knockdown significantly suppressed CML cell proliferation and hsa{sub c}irc{sub 0}080145 regulated CML cell proliferation by acting as an miR-29b sponge.

• OSTI ID: 23134185
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 504; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
MYELOID LEUKEMIA
PATHOGENESIS
PORIFERA
RNA