MYPT1 is targeted by miR-145 inhibiting viability, migration and invasion in 2D and 3D HeLa cultures

Journal Article · Sat Dec 15 04:00:00 EST 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.11.039· OSTI ID:23134173

González-Torres, Alejandro; Bañuelos-Villegas, Evelyn Gabriela; Martínez-Acuña, Natalia ^[1]; Sulpice, Eric; Gidrol, Xavier ^[2]

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1. Laboratorio de Terapia Génica, Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del I.P.N., Av. I.P.N. 2508, Ciudad de México, 07360 (Mexico)
2. University of Grenoble Alpes, CEA, INSERM, BIG-BGE U1038, 38000, Grenoble (France)

Highlights: • miR-143/145 affect viability, migration and invasion on monolayer and spheroid cultures. • miR-145 produces a stronger inhibitory effect and increases pMLC levels through MYPT1 targeting. • MYPT1 silencing by siRNAs reproduces miR-145 effects. The miR-143/145 cluster is down-regulated in cervical tumor cells suggesting a role in tumorigenesis including cytoskeleton remodeling, a key event for tumor progression. The aim of the present work was to determine the role of miR-143/145 in the modulation of the myosin regulator phospho-myosin light chain (pMLC). HeLa monolayer and tridimensional cultures were transfected with miR-143 or miR-145 mimics inhibiting cell viability, proliferation, migration and invasion, mainly through miR-145. MiR-145 transfection increased pMLC levels by targeting the MYPT1 subunit of the regulatory myosin phosphatase. MYPT1 knockdown by siRNAs reproduced miR-145 effects suggesting miR-145 as a tumor suppressor through MYPT1 targeting, leading to a subsequent increase of pMLC levels with implications for cervical cell viability, migration and invasion.

• OSTI ID: 23134173
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1-4 Vol. 507; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
MICROTUBULES
MYOSIN
NEOPLASMS
PHOSPHATASES
TUMOR CELLS