Ferricrocin, the intracellular siderophore of Trichoderma virens, is involved in growth, conidiation, gliotoxin biosynthesis and induction of systemic resistance in maize
Journal Article
·
· Biochemical and Biophysical Research Communications
- Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, 77840 (United States)
- USDA ARS, Southern Plains Agricultural Research Center, College Station, TX (United States)
- Research Area Biochemical Technology, Institute of Chemical and Biological Engineering, TU Wien, 1060, Vienna (Austria)
- Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln (Austria)
Highlights: • TvTex10 deletion increases fungal growth rate and sensitivity to oxidative stress. • Mutants demonstrate decreased conidiation and gliotoxin production. • Root colonization is increased in mutant treated plants. • Induced systemic resistance in treated plants is reduced compared to the wild type. Fungal siderophores are known to be involved in iron acquisition and storage, as well as pathogenicity of mammals and plants. As avirulent plant symbionts, Trichoderma spp. colonize roots and induce resistance responses both locally and systemically. To study the role of intracellular siderophore(s) in Trichoderma-plant interactions, we have obtained mutants in a non-ribosomal peptide synthetase, TvTex10, that was predicted to be involved in intracellular siderophore(s) biosynthesis. This gene has a detectable basal level of expression and is also upregulated under iron-deplete conditions. This is unlike two other siderophore-encoding genes, which are tightly regulated by iron. Disruption of tex10 gene using homologous recombination resulted in mutants with enhanced growth rate, reduced conidiation and hyper-sensitivity to oxidative stress as compared to wildtype strain. The mutants also produced reduced levels of gliotoxin and dimethyl gliotoxin but have enhanced ability to colonize maize seedling roots. The mutants were also impaired in induction of induced systemic resistance (ISR) in maize against the foliar pathogen Cochliobolus heterostrophus.
- OSTI ID:
- 23134133
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 505; ISSN BBRCA9; ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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