Sterol regulatory element binding protein-1: Molecular cloning, tissue distribution and gene expression level in response to nutritional regulation in mud crab, Scylla paramamosain
- College of Marine Sciences, South China Agricultural University, Guangzhou, 510642 (China)
- Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, 515063 (China)
Highlights: • The complete SREBP-1 cDNA sequence was cloned from the hepatopancreas of mud crab based on the RACE technology. • Tissue distribution analysis revealed that SREBP-1 transcripts were widely distributed in various organs, with higher mRNA levels in the eyestalk and cranial ganglia. • The expression level of SREBP-1 mRNA were up-regulated in proportion to the replacement of dietary fish oil with soybean oil. In the present study, SREBP-1 cDNA was cloned from the hepatopancreas of mud crab (Scylla paramamosain) and characterized by performing rapid-amplification of cDNA ends. The 3361bp long full-length cDNA encodes a polypeptide with 1039 amino acids. Tissue distribution analysis revealed that SREBP-1 transcripts were widely distributed in various organs, with higher mRNA levels in the eyestalk and cranial ganglia. Further, expression level of SREBP-1 mRNA were up-regulated in proportion to the replacement of dietary fish oil (FO) with soybean oil (SO). These results may contribute to better understanding of the long-chain polyunsaturated fatty acids (LC-PUFA) biosynthetic pathway and regulation mechanism in mud crab.
- OSTI ID:
- 23134123
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 505, Issue 3; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Copper toxicity in the crab, Scylla serrata, copper levels in tissues and regulation after exposure to a copper-rich medium
In vivo promoter analysis on refeeding response of hepatic sterol regulatory element-binding protein-1c expression