A Prdm8 target gene Ebf3 regulates multipolar-to-bipolar transition in migrating neocortical cells

Journal Article · Sun Jan 14 23:00:00 EST 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2017.11.021· OSTI ID:23134073

Iwai, Ryota ^[1]; Tabata, Hidenori ^[2]; Inoue, Mayuko; Nomura, Kei-ichiro ^[3]; Okamoto, Tadashi; Ichihashi, Masamitsu ^[4]; Nagata, Koh-ichi ^[2]; Mizutani, Ken-ichi ^[1]

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1. Laboratory of Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Kobe Gakuin University, Kobe 650-8586 (Japan)
2. Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, Aichi 480-0392 (Japan)
3. Laboratory of Neural Differentiation, Graduate School of Brain Science, Doshisha University, Kyoto 6190225 (Japan)
4. Laboratory of Biochemistry, Graduate School of Pharmaceutical Sciences, Kobe Gakuin University, Kobe 650-8586 (Japan)

Highlights: • Precise control of neuronal migration is essential for the development of the neocortex. • We identified helix-loop-helix transcription factor Ebf3 as a Prdm8 target gene. • Ebf3 is a key regulator of neuronal migration via multipolar-to-bipolar transition. • Ebf3 knockdown cells exhibited severe defects in the formation of leading processes. • Ebf3 in multipolar-to-bipolar transition via positive feedback regulation of NeuroD1. Precise control of neuronal migration is essential for the development of the neocortex. However, the molecular mechanisms underlying neuronal migration remain largely unknown. Here we identified helix-loop-helix transcription factor Ebf3 as a Prdm8 target gene, and found that Ebf3 is a key regulator of neuronal migration via multipolar-to-bipolar transition. Ebf3 knockdown cells exhibited severe defects in the formation of leading processes and an inhibited shift to the locomotion mode. Moreover, we found that Ebf3 knockdown represses NeuroD1 transcription, and NeuroD1 overexpression partially rescued migration defects in Ebf3 knockdown cells. Our findings highlight the critical role of Ebf3 in multipolar-to-bipolar transition via positive feedback regulation of NeuroD1 in the developing neocortex.

• OSTI ID: 23134073
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 495; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
GENES
REGULATIONS
STEM CELLS
TRANSCRIPTION
TRANSCRIPTION FACTORS