Promoting hepatogenic differentiation of human mesenchymal stem cells using a novel laminin-containing gelatin cryogel scaffold

Journal Article · Sat Dec 15 04:00:00 EST 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.10.121· OSTI ID:23134047

Mohammadpour, Alemeh ^[1]; Arjmand, Sareh ^[2]; Tavana, Hossein ^[3]; Kabir-Salmani, Maryam ^[1]

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1. Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran (Iran, Islamic Republic of)
2. Protein Research Center, Shahid Beheshti University, G. C, Tehran (Iran, Islamic Republic of)
3. Department of Biomedical Engineering, The University of Akron, Akron, OH, 44325 (United States)

Highlights: • The gelatin cryogel was optimized for stability in cell culture medium during stem cell differentiation. • The optimized gelatin cryogel improved with the laminin, the main component of basal lamina. • The novel scaffold with laminin efficiently enhanced differentiation of mesenchymal stem cells toward hepatocyte lineage. Liver transplantation is the only definitive treatment currently available for acute and chronic liver failure. However, this approach has been restricted by complications including rejection and infection. Tissue engineering approaches using stem cell-derived functional hepatic cells offer a potential alternative. Using biologically compatible scaffolds is an important complementary key to achieve optimal construct for hepatic replacement. In the present study, to optimize the differentiation of human adipose-derived mesenchymal stem cells (ADMSCs) toward hepatocyte-like cells, a previously described gelatin cryogel was optimized and improved by laminin, the major component of basal lamina. The ADMSCs seeded on the scaffold displayed increased attachment in the presence of laminin and the MTT assay showed good compatibility for cell proliferation. The differentiation of stem cells were evaluated using glycogen staining, urea secretion measurement, hepatocyte specific cell surface analysis and gene expression analysis. The results of tests indicated that laminin protein and gelatin cryogel 3D scaffold, each on its own, enhanced hepatogenic differentiation of ADMSCs. However, when laminin immobilized on the gelatin cryogel surface, the differentiation was promoted significantly and the resulting cells showed striking similarity to HepG2 in terms of expressing studied hepatocyte markers.

• OSTI ID: 23134047
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1-4 Vol. 507; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL CULTURES
CELL DIFFERENTIATION
CULTURE MEDIA
GLYCOGEN
LIVER CELLS
STEM CELLS
UREA