Long-term fermented soybean paste improves metabolic parameters associated with non-alcoholic fatty liver disease and insulin resistance in high-fat diet-induced obese mice
- School of Life Science, Handong Global University, Pohang, Gyungbuk (Korea, Republic of)
- Department of Advanced Green Energy and Environment, Handong Global University, Pohang, Gyungbuk (Korea, Republic of)
- Research & Development Team, Advanced Bio Convergence Center, Pohang Technopark Foundation, Pohang, Gyungbuk (Korea, Republic of)
Highlights: • LFSP, but not SFSP, reverses HFD-induced NAFLD and insulin resistance in mice. • LFSP suppresses mesenteric fat lipolysis and hepatic lipid uptake. • LFSP enhances insulin sensitivity and adiponectin-mediated anti-inflammatory action. • LFSP suppresses HFD-induced gut permeability leading to attenuated chronic inflammation. • LFSP-derived Bacillus strains attenuates HFD-induced obesity and glucose intolerance. Recently, Korean traditional fermented soybean paste, called Doenjang, has attracted attention for its protective effect against diet-related chronic diseases such as obesity and type 2 diabetes. Long-term fermented soybean pastes (LFSPs) are made by fermentation with naturally-occurring microorganisms for several months, whereas short-term fermented soybean pastes (SFSPs) are produced by shorter-time fermentation inoculated with a starter culture. Here, we demonstrate that administration of LFSP, but not SFSP, protects high-fat diet (HFD)-fed obese mice against non-alcohol fatty liver disease (NAFLD) and insulin resistance. LFSP suppressed body weight gain in parallel with reduction in fat accumulation in mesenteric adipose tissue (MAT) and the liver via modulation of MAT lipolysis and hepatic lipid uptake. LFSP-treated mice also had improved glucose tolerance and increased adiponectin levels concomitantly with enhanced AMPK activation in skeletal muscle and suppressed expression of pro-inflammatory cytokines in skeletal muscle and the liver. LFSP also attenuated HFD-induced gut permeability and lowered serum lipopolysaccharide level, providing an evidence for its probiotic effects, which was supported by the observation that treatment of a probiotic mixture of LFSP-originated Bacillus strains protected mice against HFD-induced adiposity and glucose intolerance. Our findings suggest that the intake of LFSP, but not SFSP, offers protection against NAFLD and insulin resistance, which is an effect of long-term fermentation resulting in elevated contents of active ingredients (especially flavonoids) and higher diversity and richness of Bacillus probiotic strains compared to SFSP.
- OSTI ID:
- 23127504
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 495, Issue 2; Other Information: Copyright (c) 2017 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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