Down-regulation of lncRNA MEG3 promotes hypoxia-induced human pulmonary artery smooth muscle cell proliferation and migration via repressing PTEN by sponging miR-21

Journal Article · Mon Jan 15 04:00:00 EST 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2017.11.185· OSTI ID:23127472

Zhu, Boqian ^[1]; Gong, Yaoyao ^[2]; Yan, Gaoliang; Wang, Dong; Qiao, Yong ^[1]; Wang, Qingjie ^[3]; Liu, Bo; Hou, Jiantong; Li, Ruifeng; Tang, Chengchun ^[1]

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1. Department of Cardiology, Zhongda Hospital, Southeast University, Nanjing (China)
2. Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing (China)
3. Department of Cardiology, Changzhou Second Hospital of Nanjing Medical University, Changzhou (China)

Highlights: • Hypoxia induced cell proliferation and migration in PASMCs. • MEG3 expression was down-regulated in PASMCs hypoxia cell models. • MEG3 exerts its role via miR-21/PTEN axis in PASMCs hypoxia cell models. Hypoxia-induced pulmonary hypertension is a life-threatening disease arising from a progressive increase in pulmonary vascular resistance, irreversible pulmonary vascular remodeling and resulting in right ventricular failure. Recent studies suggested that pulmonary artery smooth muscle cell proliferation and migration played an important role in the pathogenesis of hypoxia-induced pulmonary hypertension. However, the mechanisms of hypoxia-induced pulmonary hypertension are complicated and largely unclear. In this study, we discovered that lncRNA MEG3 was down-regulated in human pulmonary artery smooth muscle cell in hypoxia, and inhibition of MEG3 promoted the cell proliferation and cell migration in both normal and hypoxia condition. Further study demonstrated that MEG3 exerted its function via regulation of miR-21 expression in both normal and hypoxia condition. In addition, we displayed the modulation of PTEN by miR-21 and their role in hypoxia. Ultimately, our study illustrated that MEG3 exerts its role via miR-21/PTEN axis in human pulmonary artery smooth muscle cell under both normal and hypoxia conditions.

• OSTI ID: 23127472
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 495; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANOXIA
ARTERIES
CELL PROLIFERATION
HUMAN POPULATIONS
HYPERTENSION
PATHOGENESIS